Protein Info for HMPREF1058_RS02650 in Phocaeicola vulgatus CL09T03C04

Annotation: CRISPR-associated protein Cas4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 TIGR00372: CRISPR-associated protein Cas4" amino acids 11 to 202 (192 residues), 109.7 bits, see alignment E=7.2e-36 PF01930: Cas_Cas4" amino acids 105 to 201 (97 residues), 60 bits, see alignment E=1.6e-20

Best Hits

KEGG orthology group: K07464, putative RecB family exonuclease (inferred from 94% identity to bhl:Bache_3083)

Predicted SEED Role

"CRISPR-associated RecB family exonuclease Cas4"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9TXK4 at UniProt or InterPro

Protein Sequence (221 amino acids)

>HMPREF1058_RS02650 CRISPR-associated protein Cas4 (Phocaeicola vulgatus CL09T03C04)
MYNEDDMLMLSGIQHFRFCPRQWALIHIEQQWNDNRLTIEGQILHKHVDDPFYRQKCGDQ
ITLRAVNIASRELGLYGISDAIELLPSSSFENTILHPNYPGRWQPVAVEYKHGKPKRNEV
DEVQLAAQIMCIEEMYAIHISYGSFFYGELRHRVNVDITEELRNIVRQCARDMHDIFSKA
VIPKAEYGKHCDKCSLKDICMPEMVNNCTSVDNYLTKNLYL