Protein Info for HMPREF1058_RS02645 in Phocaeicola vulgatus CL09T03C04

Annotation: type I-C CRISPR-associated endonuclease Cas1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 TIGR03640: CRISPR-associated endonuclease Cas1, subtype I-C/DVULG" amino acids 3 to 338 (336 residues), 447.1 bits, see alignment E=4.2e-138 TIGR00287: CRISPR-associated endonuclease Cas1" amino acids 7 to 334 (328 residues), 283.4 bits, see alignment E=2.3e-88 PF01867: Cas_Cas1" amino acids 8 to 294 (287 residues), 339.6 bits, see alignment E=6.6e-106

Best Hits

Swiss-Prot: 48% identical to CAS1A_CHLTE: CRISPR-associated endonuclease Cas1 1 (cas1-1) from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)

KEGG orthology group: None (inferred from 96% identity to bhl:Bache_3082)

Predicted SEED Role

"CRISPR-associated protein Cas1" in subsystem CRISPRs

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZHL9 at UniProt or InterPro

Protein Sequence (340 amino acids)

>HMPREF1058_RS02645 type I-C CRISPR-associated endonuclease Cas1 (Phocaeicola vulgatus CL09T03C04)
MRKLLNTLYVTTPEAYLSKDGLNVVISVQQEEVFRIPVINIEGIVTFGYMGASPGVMKLC
SDNGISLTFLSPQGRFISRVQGATKGNVLLRKKQYQLSDDASWSLHVVRLMIGGKIQNYR
NILRRYIRDYGENEDINRAVQVLERAKRDALKAQDKTELIGYEGMASNAYFEVLPILILN
QKTDFPFHGRNRRPPKDAVNAMLSFAYTLIANDVAAALETVGLDPYVGFLHTLRPGRTSL
ALDMMEELRAYLGDRFILSLINKRQISVKDFLFQGDNGVVMTDKGKKTFITAWQARKREV
IIHPYLNEKVEIGLLPYVQAMLMARYIRQDIDDYPVFLIK