Protein Info for HMPREF1058_RS01670 in Phocaeicola vulgatus CL09T03C04

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1162 TIGR00392: isoleucine--tRNA ligase" amino acids 8 to 961 (954 residues), 1021.2 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 20 to 749 (730 residues), 386.4 bits, see alignment E=3e-119 PF09334: tRNA-synt_1g" amino acids 52 to 195 (144 residues), 20.3 bits, see alignment E=4.3e-08 amino acids 690 to 754 (65 residues), 23.1 bits, see alignment 6e-09 PF08264: Anticodon_1" amino acids 797 to 948 (152 residues), 114.9 bits, see alignment E=6.3e-37 PF19302: DUF5915" amino acids 967 to 1161 (195 residues), 181.7 bits, see alignment E=2.4e-57

Best Hits

Swiss-Prot: 87% identical to SYI_BACTN: Isoleucine--tRNA ligase (ileS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 100% identity to bvu:BVU_3059)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZDH3 at UniProt or InterPro

Protein Sequence (1162 amino acids)

>HMPREF1058_RS01670 isoleucine--tRNA ligase (Phocaeicola vulgatus CL09T03C04)
MSKKFAEHSQLNLSQVNKDVLKKWDENDVFAKSMTEREGCPSFVFYEGPPSANGMPGIHH
VMARTIKDTFCRYKTMKGFQVKRKAGWDTHGLPVELGVEKALHITKEDIGKTISVAEYNA
ACRKDVMKFTKEWTDLTHKMGYWVDLENPYITYDNRYIETLWWLLKQLYNKGLLYKGYTI
QPYSPAAGTGLSSHELNQPGCYRDVKDTTVVGQFKMKNPKPEMAEWGTPYFLAWTTTPWT
LPSNTALCVGPKIDYVAVQTYNGYTGEKMTVVLAKALLYTHFNKKAEELALEDYKPGDKL
IPFKVVGEYKGPDLVGMEYEQLIPWVKPVTVDENGKWQEAAGEAFRVIPGDYVTTEDGTG
IVHIAPTFGADDAFVARAAGIPSLFMINKKGETRPMVDLTGKFYLLDELDEAFVKECVDV
EKYKEYQGRWVKNAYDPQFTVDGKYDEKAAAAAESLDIYICMMMKAANKAFKIEKHVHNY
PHCWRTDKPVLYYPLDSWFIRSTAAKERMMELNKTINWKPESTGTGRFGKWLENLNDWNL
SRSRYWGTPLPIWRSEEGEELCIGSVEELYNEIEKSIAAGFMTVNPYKEKGFVPGEYNGE
NYDKIDLHRPYVDDIILVSESGKPMKREADLIDVWFDSGAMPYAQLHYPFENKEIVDNRT
YYPADFIAEGVDQTRGWFFTLHAIATMVFDSVAYKNVISNGLVLDKNGNKMSKRLGNAVD
PFGAIEQYGSDPLRWYMITNSSPWDNLKFDTDGVDEVRRKFFGTLYNTYSFFALYANVDG
FAYQEAEVPVNERPEIDRWILSVLNSLVKNVDACYNDYEPTKAGRLITDFVNDNLSNWYV
RLNRKRFWGNAMSQDKLSAYQTLYTCLETVARLMAPIAPFYADRLYTDLIAATGRDTVVS
VHLAKFPECNEALIDGELEARMQMAQDVTSMVLALRRKVNIKVRQPLQCIMVPVVDEEQK
MHIEAVKDLIMSEVNVKEIKFVDGAAGVLVKKVKCDFKKLGPKFGKQMKAVAAAVAGMSQ
EAIAELEKNGKYTFVLDGAEAVIETTDVEIFSEDIPGWLVANEGKLTVALEVTVTEELRR
EGVARELVNRIQNIRKSSGFEITDKIKVALSKNPQTDDAVNEYNTYICNQVLANSLELVD
EVKNGTELNFDDFSLYVSVVKE