Protein Info for HMPREF1058_RS01425 in Phocaeicola vulgatus CL09T03C04

Annotation: sirohydrochlorin cobaltochelatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF06180: CbiK" amino acids 50 to 232 (183 residues), 101.6 bits, see alignment E=2.4e-33 amino acids 267 to 380 (114 residues), 36.7 bits, see alignment E=1.6e-13

Best Hits

KEGG orthology group: K02190, sirohydrochlorin cobaltochelatase [EC: 4.99.1.3] (inferred from 97% identity to bvu:BVU_3107)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase CbiK (EC 4.99.1.3)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 4.99.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I8ZF75 at UniProt or InterPro

Protein Sequence (398 amino acids)

>HMPREF1058_RS01425 sirohydrochlorin cobaltochelatase (Phocaeicola vulgatus CL09T03C04)
MKQFKYYLMAAFVAAVTCTGFSSCSDNDDEESTVNPAVDVVSKTKQHDTAILLCTFGSTY
NESLTVYDDIIEDFKSKFPNTDIYMSFTSRTCIRRVEASTGIARYELDQWLKAIGNAGYK
RVAVQSLHVIPGEEYLSLMNTDVKKYFMIQWYPHIDVLKGANLLSSAEDTKDVAEILYKH
YESKLAGKNNIVLLMGHGNPDENYNANTKYSDMEKALQELAANNNIFVGTVDYGDMLFFP
KEIEKEPANRIPVEGFDKTQYPDCMYSKVMSYCEKNGLNPSEVNVYLAPFMSIAGDHAHN
DLWGLEAMAEDDDVSNVEINTNEYSWRERLEKLGFKVDRTFESHPIDQAGADHGIKDGCN
MKALGSYPEIRQIWVNHLYENWNDDSAWENGEDYQPEA