Protein Info for HMPREF1058_RS01020 in Phocaeicola vulgatus CL09T03C04

Annotation: bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase" amino acids 6 to 202 (197 residues), 237.4 bits, see alignment E=1.1e-74 PF00926: DHBP_synthase" amino acids 10 to 201 (192 residues), 275.8 bits, see alignment E=1.6e-86 PF00925: GTP_cyclohydro2" amino acids 212 to 376 (165 residues), 242.8 bits, see alignment E=1.4e-76 TIGR00505: GTP cyclohydrolase II" amino acids 214 to 400 (187 residues), 251.4 bits, see alignment E=4.6e-79

Best Hits

Swiss-Prot: 100% identical to RIBBA_BACV8: Riboflavin biosynthesis protein RibBA (ribBA) from Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 100% identity to bvu:BVU_0143)

MetaCyc: 46% identical to GTP cyclohydrolase II (Chlamydia trachomatis)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I9TU98 at UniProt or InterPro

Protein Sequence (404 amino acids)

>HMPREF1058_RS01020 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II (Phocaeicola vulgatus CL09T03C04)
MEELKLNTIEEAIADFREGKFVIVVDDEDRENEGDLIVAAEKITPEQVNFMLKHARGVLC
APITISRCKELELPHQVDTNTSVLGTPFTVTVDKLEGCSTGVSIHDRAATIRALADPTST
PETFGRPGHVNPLYAQDKGVLRRAGHTEACIDMARLAGLYPAAALMEIMSEDGTMARLPE
LRKMADEWGLKLISIRDLIAYRLKQESLVEKGVEVDMPTEYGHFRLIPFRQKSNGLEHIA
IIKGDIKEGEPVLVRVHSSCATGDIFGSMRCDCGEQLHKALQMIEKEGKGAVVYLNQEGR
GIGLMEKMKAYKLQENGVDTVEANILLGHQADERDYGVGAQILRSIGVTQMRLLTNNPVK
RVGLESYGLSIVENVPIEITPNKYNERYLKTKKDRMGHTLHFNK