Protein Info for HMPREF1058_RS00415 in Phocaeicola vulgatus CL09T03C04

Annotation: adenylosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 12 to 447 (436 residues), 401.2 bits, E=2.6e-124
• PF00206: Lyase_1
  • amino acids 14 to 313 (300 residues), 175.3 bits, E=2.2e-55
• PF08328: ASL_C
  • amino acids 331 to 446 (116 residues), 161.2 bits, E=9.6e-52

Best Hits

Swiss-Prot: 60% identical to PUR8_DICDI: Adenylosuccinate lyase (purB) from Dictyostelium discoideum

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 100% identity to bvu:BVU_1626)

MetaCyc: 52% identical to adenylosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis I (5/6 steps found)
• dZTP biosynthesis (4/5 steps found)
• inosine-5'-phosphate biosynthesis III (4/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Sequence Analysis Tools

See I9IHR5 at UniProt or InterPro

Protein Sequence (448 amino acids)

>HMPREF1058_RS00415 adenylosuccinate lyase (Phocaeicola vulgatus CL09T03C04)
MELDLLTAISPIDGRYRGKAGALASYFSEFALIKYRVQVEIEYFITLCELPLPQLKNFDH
ARFDALRAIYKNFSEADAQRIKEIESVTNHDVKAVEYFIKEEFDKLGGMEEYKEFIHFGL
TSQDINNTSVPLSIKEALEQVYYPQIEELIAQLTTYAEEWANIPMLAKTHGQPASPTRLG
KEIMVFVYRLNRQLAVLKACPVTAKFGGATGNYNAHHVAYPEYDWKAFGNQFVAEKLGLE
REEYTTQISNYDNLGAIFDAMKRINTIMIDMNRDFWQYISMEYFKQKIKAGEVGSSAMPH
KVNPIDFENAEGNLGMANAILEHLSSKLPVSRLQRDLTDSTVLRNVGVPFGHIVIAIQSS
LKGLRKLLLNEKAIYADLDNCWSVVAEAIQTILRREAYPHPYEALKALTRTNQAITEGAI
KEFIETLNVSEDIKKELRVITPHNYTGI