Protein Info for H281DRAFT_06426 in Paraburkholderia bryophila 376MFSha3.1

Annotation: ATP-dependent Lon protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 PF02190: LON_substr_bdg" amino acids 29 to 220 (192 residues), 118.4 bits, see alignment E=1.1e-37 TIGR00763: endopeptidase La" amino acids 32 to 788 (757 residues), 847.6 bits, see alignment E=5.9e-259 PF00004: AAA" amino acids 372 to 508 (137 residues), 79.1 bits, see alignment E=1.1e-25 PF07728: AAA_5" amino acids 372 to 498 (127 residues), 29.3 bits, see alignment E=1.9e-10 PF05362: Lon_C" amino acids 590 to 791 (202 residues), 267.6 bits, see alignment E=1.7e-83 PF13541: ChlI" amino acids 634 to 760 (127 residues), 27.8 bits, see alignment E=5.2e-10

Best Hits

Swiss-Prot: 89% identical to LON_PARPJ: Lon protease (lon) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 89% identity to bpy:Bphyt_5717)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (792 amino acids)

>H281DRAFT_06426 ATP-dependent Lon protease (Paraburkholderia bryophila 376MFSha3.1)
MTTDSDSDTSESGPASQAISAASAMPEETLILLPVRNAVLFPGMVLPFTAGRQQSKEDVQ
AAVRRQKPLGIVLQLDPQARDPGFDGLNTIGTVANVVRYVTSPDDGAHHLICQGVERFRL
LAPVEGLGFRAARVDLLPDTAPSNSAIDARALVLRQRAGEMIGLLPNAGVDLVRALDAIE
APGLLADTIAGLLDIPPERKQEILETLDVCKRLDKVLDAVAARIDVLRLSQEIGEQTRGR
LDQRQREMMLREQLRTIQNELGETVESREEVRKLNDAIQAAQMPPDVESHARKELDRLER
MPEASSEYSISVSYLEWVTELPWQLPPQTPIDIARARQTLDEAHFGLDKVKRRILEYLGV
RKLNPGGKAPILCFLGPPGVGKTSLGQSIARALERPFVRVGLGGVHDEAEIRGHRRTYIG
AMPGNIIQAIRKAGARNCVMMLDELDKLGQGIHGDPAAAMLEVLDPEQNASFRDNYLAVP
FDLSAVVFVATANQAEGIPGPLRDRMEILDLPGYTEAEKFQIARRFLVPRQLEACGLTAA
QCELPDETLRAIIRDYTREAGVRALERQIGAIFRYVALRVAEDPSISERVEPDRLPSILG
QRRFESEVAMRTSLPGVVTGLAWTPVGGDLLFIEASSTPGSGRLVLTGQLGDVMKESVQA
ALTLVKSRCESLHIDCSSFDKRDIHVHVPAGAVAKDGPSAGVAMFVAIASLFMGRAVRSD
CAVTGEISLRGIVLPVGGIKEKLLAALRGGISTVLLPARNAADVEDVPVDARNQMRFVLL
ETVDDAMRELIQ