Protein Info for H281DRAFT_06393 in Paraburkholderia bryophila 376MFSha3.1

Annotation: N-carbamoyl-L-amino-acid hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01879: amidase, hydantoinase/carbamoylase family
  • amino acids 20 to 415 (396 residues), 440.1 bits, E=3.9e-136
• PF01546: Peptidase_M20
  • amino acids 91 to 415 (325 residues), 91.7 bits, E=6.3e-30
• PF07687: M20_dimer
  • amino acids 222 to 317 (96 residues), 27.7 bits, E=2.3e-10

Best Hits

Swiss-Prot: 46% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: None (inferred from 88% identity to bug:BC1001_5099)

MetaCyc: 46% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

• thymine degradation (3/3 steps found)
• uracil degradation I (reductive) (3/3 steps found)
• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.6

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Protein Sequence (432 amino acids)

>H281DRAFT_06393 N-carbamoyl-L-amino-acid hydrolase (Paraburkholderia bryophila 376MFSha3.1)
MQVHESQATEAVNRLRVDGERLWNSLMQLAKIGATEKGGVCRLALTELDRIARDQVVSWA
KEIGCTVRIDAIGNIFARRAGLRDDLPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVLRTL
ADANVTTLAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLEHALAQQDRDGVSVRDALTA
IGYVGENTKPHTVGAYFEAHIEQGPVLEANDKTIGIVQGALGQRWYDVTVHGMEAHAGPT
PMELRRDALLVAADLIHAVNRIALDHAPHGRGTVGWVDVHPNSRNVIPGRVKLTVDLRAA
DDTTLTAMDSALRDACTVASEKAGIAVDVEPVVYFAPQPFATELVAAVKHGAEMLGLSSM
DVISGAGHDAVYLARVAPAAMIFVPCKDGISHNEIEDARADHLEAGCNVLLHAMLRAAQV
VQTAPMSEGSNV