Protein Info for H281DRAFT_06321 in Paraburkholderia bryophila 376MFSha3.1

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 TIGR00396: leucine--tRNA ligase" amino acids 8 to 863 (856 residues), 1069.2 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 16 to 223 (208 residues), 90.8 bits, see alignment E=2.1e-29 amino acids 420 to 575 (156 residues), 32.4 bits, see alignment E=1.1e-11 amino acids 622 to 662 (41 residues), 27.8 bits, see alignment 2.8e-10 PF09334: tRNA-synt_1g" amino acids 38 to 171 (134 residues), 68.9 bits, see alignment E=9.2e-23 PF13603: tRNA-synt_1_2" amino acids 220 to 406 (187 residues), 229.4 bits, see alignment E=6.2e-72 PF08264: Anticodon_1" amino acids 703 to 825 (123 residues), 66.7 bits, see alignment E=5.5e-22

Best Hits

Swiss-Prot: 87% identical to SYL_BURCJ: Leucine--tRNA ligase (leuS) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 87% identity to bcj:BCAL3373)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MG07 at UniProt or InterPro

Protein Sequence (863 amino acids)

>H281DRAFT_06321 leucyl-tRNA synthetase (Paraburkholderia bryophila 376MFSha3.1)
MHEKYVPSDVESAAQGQWRAIDAYKTSETTDKPKFYCVSMLPYPSGKLHMGHVRNYTIND
VMYRYLRMNGYNVLMPMGWDAFGMPAENAAMANNVPPAKWTYDNIAYMKKQMQSMGLAID
WSREVATCSPDYYRWNQWLFLKMLEKGIAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
ALVEKREIPMYYMRITQYADELLNDLEGLGWPERVKIMQQNWIGKSFGVNFGFPYEIDGE
QKLLRVFTTRADTIMGVTFAAVAAEHPLATRLAQDKPELQAFIDECKRGGVAEADVATME
KKGMRTGFFVKHPLTQEPVEVWIGNYVLMTYGEGAVMGVPAHDERDFAFAKKYGLPIKQV
VAVEGKEFSTQAWEEWYGEKGGTLVNSGKYDGLNYEQAVDAIAADLKALGLGDKQITWRL
RDWGVSRQRYWGTPIPIIHCPECGDVPVPEKDLPVVLPEDLVPDGTGNPLAKSEAFVNCT
CPTCGSAAKRETDTMDTFVDSSWYFYRYASPGAPTMVDERTDYWAPMDQYIGGIEHAILH
LLYSRFWAKVMRDLGLVKFGEPAKNLLTQGMVLNETYYREDATGKKTWYNPADVTVSFDD
KGRPVGAVLNSDGLPVVLGGVEKMSKSKNNGVDPQSLIDQHGADTARLFVMFAAPPEQQL
EWSGSGVEGASRFLRRVWSFGHANEAALRAGGQFDAAQLADADKLLRREIYSVLKQADFD
YQRLQYNTVVSAAMKMLNALDSAKGAQPAVLRETYGVMLRVLYPVVPHLTFQLWQELGYA
DEFGSLLDAPWPKVDEKALEQSEIELVLQVNGKVRGALTVSKDASRESIEQLAAAHEMVA
KFSEGKAPKKIVVVPGRLVNVVV