Protein Info for H281DRAFT_06072 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3-isopropylmalate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00169: 3-isopropylmalate dehydrogenase
  • amino acids 2 to 350 (349 residues), 486.1 bits, E=2.9e-150
• PF00180: Iso_dh
  • amino acids 2 to 351 (350 residues), 450.8 bits, E=1.8e-139

Best Hits

Swiss-Prot: 91% identical to LEU3_CUPNJ: 3-isopropylmalate dehydrogenase (leuB) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K00052, 3-isopropylmalate dehydrogenase [EC: 1.1.1.85] (inferred from 99% identity to bgf:BC1003_4594)

Predicted SEED Role

"3-isopropylmalate dehydrogenase (EC 1.1.1.85)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 1.1.1.85)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• butanol and isobutanol biosynthesis (engineered) (4/8 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.85

Sequence Analysis Tools

See A0A2Z5MKG6 at UniProt or InterPro

Protein Sequence (355 amino acids)

>H281DRAFT_06072 3-isopropylmalate dehydrogenase (Paraburkholderia bryophila 376MFSha3.1)
MKIAVLPGDGIGPEIVKEAVKVLNVLGEKFELEEAPVGGAGYEAKGHPLPDSTLALAKEA
DAILFGAVGDWKYDKLERALRPEQAILGLRKHLQLFANFRPAICYPQLTGASSLKEEIVS
GLDILIVRELNGDIYFGAPRGVREAPDGLFAGSKEGFDTMRYSEPEVRRIAHVAFQAAQK
RGKKLTSVDKANVLETSQFWKDVMIDVSKEYADVELSHMYVDNAAMQLVKAPKSFDVIVT
GNMFGDILSDEAAMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGVANPLATILSA
AMMLRYSLNKAEQADRIENAVKKVLEQGYRTGDILTSGCKQVGTVAMGDAVVAAL