Protein Info for H281DRAFT_06059 in Paraburkholderia bryophila 376MFSha3.1
Annotation: O-succinylhomoserine sulfhydrylase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to METZ_PSEAE: O-succinylhomoserine sulfhydrylase (metZ) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K10764, O-succinylhomoserine sulfhydrylase [EC: 2.5.1.-] (inferred from 96% identity to bxe:Bxe_B2873)MetaCyc: 57% identical to O-succinyl-L-homoserine sulfhydrylase (Pseudomonas aeruginosa PAO1)
Cystathionine gamma-synthase. [EC: 2.5.1.48]
Predicted SEED Role
"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)
MetaCyc Pathways
- aspartate superpathway (22/25 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
- L-homocysteine biosynthesis (2/2 steps found)
- L-methionine biosynthesis III (3/4 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
- L-methionine biosynthesis II (4/6 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
- L-methionine biosynthesis I (2/5 steps found)
- homocysteine and cysteine interconversion (1/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Cysteine metabolism
- Methionine metabolism
- Porphyrin and chlorophyll metabolism
- Riboflavin metabolism
- Selenoamino acid metabolism
- Sulfur metabolism
- Terpenoid biosynthesis
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.-, 2.5.1.48, 2.5.1.49
Use Curated BLAST to search for 2.5.1.- or 2.5.1.48 or 2.5.1.49
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5MM38 at UniProt or InterPro
Protein Sequence (396 amino acids)
>H281DRAFT_06059 O-succinylhomoserine sulfhydrylase (Paraburkholderia bryophila 376MFSha3.1) MDDSLNFDTLAVRSGTVRSDFNEHSEAIFLTSSFVFASAADAAERFKNSEDYYTYSRFTN PTVSMFQDRLAALEGGEACMATASGMAAIMSVVMSTLQAGDHLVSSQALFGSTLGMFSQI FSKFGITTTFVDPTNLDAWKNAVRPETKMFFLETPSNPLTEVADIEAISKIAKAANALFV VDNCFCSPALQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKKFIMEKVFPFVRSAGP TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDDHPSVKRVFYPGLESHPQHALAMR QQKAGGAIVSFELKGDTPEQMRANAWRVIDSTKICSITGNLGDTRTTITHPATTTHGRVT PEARAAAGITEGLIRLAVGLENAADIRADLERGLAG