Protein Info for H281DRAFT_05723 in Paraburkholderia bryophila 376MFSha3.1
Updated annotation (from data): bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174)
Rationale: Specifically important for utilization of phenylacetate. This is probably the bifunctional thiolase in the aerobic phenylacetyl-CoA degradation pathway (also known as PaaJ).
Original annotation: acetyl-CoA acyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to CATF_ACIAD: Beta-ketoadipyl-CoA thiolase (catF) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
KEGG orthology group: None (inferred from 94% identity to bug:BC1001_3126)MetaCyc: 80% identical to 3-oxoadipyl-CoA thiolase (Cupriavidus necator H16)
3-oxoadipyl-CoA thiolase. [EC: 2.3.1.174]
Predicted SEED Role
"Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)" (EC 2.3.1.-, EC 2.3.1.9)
MetaCyc Pathways
- oleate β-oxidation (29/35 steps found)
- aromatic compounds degradation via β-ketoadipate (9/9 steps found)
- phenylacetate degradation I (aerobic) (9/9 steps found)
- superpathway of salicylate degradation (7/7 steps found)
- catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
- adipate degradation (5/5 steps found)
- superpathway of phenylethylamine degradation (9/11 steps found)
- acetyl-CoA fermentation to butanoate (6/7 steps found)
- ketolysis (3/3 steps found)
- polyhydroxybutanoate biosynthesis (3/3 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- 3-oxoadipate degradation (2/2 steps found)
- acetoacetate degradation (to acetyl CoA) (2/2 steps found)
- adipate biosynthesis (4/5 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
- 2-deoxy-D-ribose degradation II (6/8 steps found)
- (2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
- mandelate degradation to acetyl-CoA (13/18 steps found)
- 4-methylcatechol degradation (ortho cleavage) (5/7 steps found)
- benzoyl-CoA degradation I (aerobic) (5/7 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
- valproate β-oxidation (6/9 steps found)
- glycerol degradation to butanol (11/16 steps found)
- (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
- glutaryl-CoA degradation (3/5 steps found)
- ketogenesis (3/5 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
- pyruvate fermentation to butanoate (4/7 steps found)
- pyruvate fermentation to acetone (2/5 steps found)
- pyruvate fermentation to butanol I (4/8 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
- superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (5/10 steps found)
- superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
- superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
- isopropanol biosynthesis (engineered) (1/5 steps found)
- ethylmalonyl-CoA pathway (5/11 steps found)
- mevalonate pathway I (eukaryotes and bacteria) (2/7 steps found)
- mevalonate pathway II (haloarchaea) (2/7 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
- L-glutamate degradation VII (to butanoate) (5/12 steps found)
- 2-methylpropene degradation (2/8 steps found)
- isoprene biosynthesis II (engineered) (2/8 steps found)
- mevalonate pathway III (Thermoplasma) (2/8 steps found)
- mevalonate pathway IV (archaea) (2/8 steps found)
- L-tryptophan degradation III (eukaryotic) (7/15 steps found)
- 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
- L-lysine fermentation to acetate and butanoate (3/10 steps found)
- methyl tert-butyl ether degradation (3/10 steps found)
- 4-oxopentanoate degradation (2/9 steps found)
- superpathway of aerobic toluene degradation (16/30 steps found)
- benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
- crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
- toluene degradation VI (anaerobic) (4/18 steps found)
- superpathway of ergosterol biosynthesis I (6/26 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)
- superpathway of L-lysine degradation (11/43 steps found)
- superpathway of cholesterol biosynthesis (6/38 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Anthocyanin biosynthesis
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Biosynthesis of terpenoids and steroids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of unsaturated fatty acids
- Butanoate metabolism
- Carotenoid biosynthesis - General
- Diterpenoid biosynthesis
- Ether lipid metabolism
- Ethylbenzene degradation
- Fatty acid biosynthesis
- Fatty acid metabolism
- Glycerophospholipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Histidine metabolism
- Limonene and pinene degradation
- Lipopolysaccharide biosynthesis
- Lysine degradation
- Phenylalanine metabolism
- Propanoate metabolism
- Pyruvate metabolism
- Synthesis and degradation of ketone bodies
- Terpenoid biosynthesis
- Tryptophan metabolism
- Tyrosine metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.174, 2.3.1.9
Use Curated BLAST to search for 2.3.1.- or 2.3.1.174 or 2.3.1.223 or 2.3.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5MFE9 at UniProt or InterPro
Protein Sequence (400 amino acids)
>H281DRAFT_05723 bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (Paraburkholderia bryophila 376MFSha3.1) MNDAYICDAIRTPIGRYGGALKDVRADDLGAVPIKALIERNPGVDWRAVDDVIYGCANQA GEDNRNVARMSALLAGLPADAPGATINRLCGSGMDAVGTAARAIKAGEAQLMIAGGVESM TRAPFVMGKAASAFARQAEIHDTTIGWRFVNPLMKRQYGVDSMPETAENVAEQFGISRAD QDAFALVSQQKAARAQRDGTLAQEIVGVEIAQKKGDAIRVTLDEHPRETSLESLAKLKGV VRPDGTVTAGNASGVNDGACALLIASQQAADQYGLRRRARVVGMATAGVEPRIMGIGPAP ATQKLLRQLGMTLDQLDVIELNEAFASQGLAVLRMLGLRDDDPRVNPNGGAIALGHPLGA SGARLVTTALHQLERSNGRFALCTMCIGVGQGIALVIERL