Protein Info for H281DRAFT_05720 in Paraburkholderia bryophila 376MFSha3.1
Annotation: phenylacetate-CoA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to PAAK_AZOEV: Phenylacetate-coenzyme A ligase (paaK) from Azoarcus evansii
KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 96% identity to bgf:BC1003_3076)MetaCyc: 73% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]
Predicted SEED Role
"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)
MetaCyc Pathways
- phenylacetate degradation I (aerobic) (9/9 steps found)
- superpathway of phenylethylamine degradation (9/11 steps found)
- penicillin G and penicillin V biosynthesis (1/4 steps found)
- phenylacetate degradation II (anaerobic) (1/4 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (3/9 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.2.1.30
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5MGG4 at UniProt or InterPro
Protein Sequence (434 amino acids)
>H281DRAFT_05720 phenylacetate-CoA ligase (Paraburkholderia bryophila 376MFSha3.1) MTTALPLDPIETASRDELCALQLERLKWSLNHAYENSPVYRRKFDEAGVHPSELTTLADL ARFPFTTKKDLRDSYPFGMFAVPQDRISRIHASSGTTGKPTVVGYTARDIDTWANLVARS IRAAGAKPGDKVHVSYGYGLFTGGLGAHYGAERAGLTVIPFGGGQTEKQVQLIQDFRPDI IMVTPSYMLSIADELERQGVDPASCSLRIGIFGAEPWTNDMRHAIEKRMGIDAVDIYGLS EVMGPGVASECVETKDGPTIWEDHFYPEIIDPETGEVLPDGELGELVFTSLTKEALPIIR YRTRDLTRLLPGSARTMRRMEKITGRSDDMMIVRGVNVFPTQIEELLLKQHALAPHYQIV LTREGPLDVLTLNVEPCPETAPDTSVLNTAKQALAYDIKALIGVSAVVNVLAVNGIERSV GKARRVVDKRKSGL