Protein Info for H281DRAFT_05639 in Paraburkholderia bryophila 376MFSha3.1

Annotation: thiazole-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 88% identical to THIG_BURTA: Thiazole synthase (thiG) from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)

KEGG orthology group: K03149, thiamine biosynthesis ThiG (inferred from 98% identity to bpy:Bphyt_3573)

MetaCyc: 44% identical to thiazole synthase (Bacillus subtilis subtilis 168)
THIAZOLSYN2-RXN [EC: 2.8.1.10]

Predicted SEED Role

"Thiazole biosynthesis protein ThiG" in subsystem Thiamin biosynthesis

MetaCyc Pathways

superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MI94 at UniProt or InterPro

Protein Sequence (273 amino acids)

>H281DRAFT_05639 thiazole-phosphate synthase (Paraburkholderia bryophila 376MFSha3.1)
MQMTTPQAADALTLYGQPFASRVLLGTSRYPSLQSLSDSIDAARPGMVTVALRRQMNEGG
AEAGFFDLLKHHGVPLLPNTAGCLTVGEAVATAHMAREIFETEWIKLELIGDDYTLQPDP
VGLIEAAAKLVKDGFKVLPYCTEDLVIGRRLLDAGCEALMPWGAPIGTGKGVINPYGLRV
LRERLPDVPLIVDAGLGVPSHAAQVMEWGFDGVLLNTAVSQATHPDAMARAFALGVEAGR
QAFLAGPMAERESAHASTPVVGMPFWHQDGSAA