Protein Info for H281DRAFT_05477 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 PF19291: TREH_N" amino acids 3 to 150 (148 residues), 71.1 bits, see alignment E=8.3e-24 PF00723: Glyco_hydro_15" amino acids 219 to 578 (360 residues), 201.4 bits, see alignment E=2.6e-63

Best Hits

KEGG orthology group: None (inferred from 91% identity to bgf:BC1003_0611)

Predicted SEED Role

"Glucoamylase (EC 3.2.1.3)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.3

Use Curated BLAST to search for 3.2.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (591 amino acids)

>H281DRAFT_05477 Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family (Paraburkholderia bryophila 376MFSha3.1)
MSRPIEDYALLGDGETAALLSSDGSIDWLCWPRFDDDACFAALLGTADNGHWMLSPVAAI
KGHARRYQRDTLIMETDFAVQDGAVRVTDFMPVGDGPSSVIRIVTGLHGVVDMDSALRLR
FNYGELAPLLERRDGAVEARIGPDCVVLRTDAHIELAAGTIRSRFTVKAGDCVSFTMTCK
RAHEAPPQPLDVQHALDETQRFWRNWIASFDDSRTAWPQAVRRSLLTLKALMHRRTGAIV
AAPTTSLPEAPGGKLNWDYRYAWLRDTSFAMVALLNAGFHGEARAWRDWLLRTISIEPER
SRIMYRVDGGRHLREWTVDWLPGHRYAKPVRVGNAAAHQHQLDVLGEVIDCLDVARRGGL
DPAPHEDLIETRIVEHLEKVWRLPGAGIWEARATPRHYTYSRVMVWVALDRFVKRRQPGS
AGTATVQRLSVLRDRIRQEALREGWNEGLGTFTQYYGGEELDASLLLMPLVGFISADDPR
MASTIENIRRGLSEGGLIRRHHRNPCGPSEGTFLACSCWMADCLNLQGRTLEARQHFERV
LATANDVGLLSEQYNVQRRELAGNFPQALSHLALINTALGLSGPTLQRGGG