Protein Info for H281DRAFT_05472 in Paraburkholderia bryophila 376MFSha3.1

Annotation: L-alanine-DL-glutamate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 32 to 128 (97 residues), 62.2 bits, E=5.4e-21
• PF13378: MR_MLE_C
  • amino acids 150 to 359 (210 residues), 202.3 bits, E=8.1e-64

Best Hits

KEGG orthology group: None (inferred from 94% identity to bgf:BC1003_0616)

Predicted SEED Role

"Mandelate racemase (EC 5.1.2.2)" (EC 5.1.2.2)

MetaCyc Pathways

• mandelate degradation to acetyl-CoA (13/18 steps found)
• 4-hydroxymandelate degradation (2/6 steps found)
• mandelate degradation I (1/5 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (18/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Toluene and xylene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.2.2

Protein Sequence (368 amino acids)

>H281DRAFT_05472 L-alanine-DL-glutamate epimerase (Paraburkholderia bryophila 376MFSha3.1)
MHLTHREAPVESIRTGAYTIPTDRPEADGTYAWQSTTLVVAEVTAAGKTGLGYTYNDAAT
AQFIEATLASAIMGEDAWNVDALWLRMQQRVRNIGRSGIAATAISALDCALWDVKARLLD
MPLARLLGAARSHIPLYGSGGFTTYTDDQIRDQLGRWVHEDGCRWVKIKIGTESVRDPHR
VQVARDAIGAQAGLFVDANGALDRKQALFYAQEFAQYGVEWFEEPVSSDDVAGLALLRDA
LPAGMELAAGEYGYTLDDFRVLLANEAIDVLQADVTRCGGITGFLRVAALCDAYHVPLSA
HCAPALHLHVACAAPRLRHQEWFHDHVRIESMLFDGAPRARNGAIAPDWSRPGCGLDFKH
ADAHRYAV