Protein Info for H281DRAFT_05446 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Nitrate/nitrite transporter NarK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details amino acids 62 to 83 (22 residues), see Phobius details amino acids 95 to 113 (19 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 153 to 178 (26 residues), see Phobius details amino acids 190 to 210 (21 residues), see Phobius details amino acids 253 to 275 (23 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 320 to 338 (19 residues), see Phobius details amino acids 344 to 367 (24 residues), see Phobius details amino acids 379 to 399 (21 residues), see Phobius details amino acids 411 to 430 (20 residues), see Phobius details PF07690: MFS_1" amino acids 33 to 396 (364 residues), 146.7 bits, see alignment E=4.2e-47

Best Hits

Swiss-Prot: 40% identical to NICT_PSEPK: Putative metabolite transport protein NicT (nicT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 96% identity to bge:BC1002_5816)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (452 amino acids)

>H281DRAFT_05446 Nitrate/nitrite transporter NarK (Paraburkholderia bryophila 376MFSha3.1)
MEPTLNTPTTPSTTPERPTPLLRLVTLRIIPFLLLCWLMAYIDRVNIGFAQLQIKHDLGF
GDAIYGLGAGIFFIGYVLFEVPSNICLEKIGARKTFSRIMICWGATSSLTMLIRTPEHFY
IMRFLLGACEAGFFPGLVFYLSCWFPESYRARVISWFFVTSAFSGIVGGMMSGTILHYLD
GANGLRGWQWMFMMEGIPSIILGIVAIFYLDDSIDKARWLDAGQKVELKKLLRPEVSRKP
EKIRFRDLFGDPYVYILAIAYFTLNSGNFGLGFWLPTILKDFGIRDPFMIGMWSALPYAI
GSIGIAIISGSSDRRQERRWHLLACTVFGGLALASLTLHVQAFWLVILQLSVAIAFMYGG
VPLFWAVPFKELNTKALAGKIALVSSLGQLGGFAAPYALGLIKAATGRLDTGLIVLGALF
AAGGVLVWITARSQASATFPAQSLASAENPET