Protein Info for H281DRAFT_05400 in Paraburkholderia bryophila 376MFSha3.1

Annotation: isoquinoline 1-oxidoreductase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 749 transmembrane" amino acids 29 to 50 (22 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 24 to 49 (26 residues), 18.2 bits, see alignment (E = 1.2e-07) PF20256: MoCoBD_2" amino acids 79 to 226 (148 residues), 53.4 bits, see alignment E=2.8e-18 amino acids 602 to 697 (96 residues), 74.4 bits, see alignment E=1.2e-24 PF02738: MoCoBD_1" amino acids 346 to 576 (231 residues), 125.5 bits, see alignment E=2e-40

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 92% identity to bug:BC1001_5670)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MJ28 at UniProt or InterPro

Protein Sequence (749 amino acids)

>H281DRAFT_05400 isoquinoline 1-oxidoreductase, beta subunit (Paraburkholderia bryophila 376MFSha3.1)
MSQGLLDAQNGATSSPDASGKRGVSRRTFLKLSVTVGAAAGGGLLLGFSMPAVSQDQKAG
KSVIGGDANEAAQNGVFAPNAFIQIDTAGKVTLVIPKVEMGQGVYTSIPMLIAEELEVPL
DTVTLDHAPPDEKLFTDPLLGGQLTGGSTSIRYAWEPMRKAGATARMLLIGAAAQQWQVD
ASSCHAKSGQVIHAASNRSIGYGQLVDAAAKLPVPQNVPLKDPKDFKIIGTAVKRLDSPE
KVDGTATFGLDVRLPDMVYAAIANCPVFGGKLASVDDTNAKKIPGVRQVVKIDNAVAVIG
DHTWAAKRGLQALDIKWNEGADAKLSMKQIVDDLANASQRNGAVARKDGDVVHAFSNAKT
RVDAVYQQPFLAHATMEPINCTVHVRPDGCEIWLGSQVPTRVRDAAMAVTGLPADKIVVH
NHLIGGGFGRRLEFDMVTQAVKIGKQVSTPVKVLWTREEDIQHDMYRPYYYDKISAGLDA
NGKPLAWQHRIVGSSIMARFAPPAFKDGLDPDAVEVASDLPYDLPNQLVDYVRQEPHAIP
TAFWRGVGPTRGTFVVESFIDELAAEAKVDPVKYRRDLLGKTPRALNVLNTAAQAANWGS
AVPKGQGRGVSVMHAFGSYFAIVVDVAVDQGEVAVKRVVCAVDCGMVVNPNTVEAQVQGG
IIFGITAALYSEITIKDGRVEQNNFTDYRMLRIDETPPIDVHIVKSSEAPGGIGEPGTAA
LAPALTNAIFAATGKRLRQLPVGSQLKTA