Protein Info for H281DRAFT_05328 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 4-oxalocrotonate tautomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01361: Tautomerase
  • amino acids 4 to 57 (54 residues), 54 bits, E=1.2e-18
• PF14552: Tautomerase_2
  • amino acids 4 to 53 (50 residues), 40.4 bits, E=2.6e-14

Best Hits

Swiss-Prot: 67% identical to Y807_RALSO: Probable tautomerase RSc0807 (RSc0807) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01821, 4-oxalocrotonate tautomerase [EC: 5.3.2.-] (inferred from 97% identity to bpy:Bphyt_4872)

MetaCyc: 32% identical to 4-oxalocrotonate tautomerase homolog (Bacillus subtilis subtilis 168)
5.3.2.M1,4.1.1.68 [EC: 4.1.1.68, 5.3.2.M1]; 5.3.2.M1 [EC: 4.1.1.68, 5.3.2.M1]; 5.3.2.M1 [EC: 4.1.1.68, 5.3.2.M1]; Phenylpyruvate tautomerase. [EC: 4.1.1.68, 5.3.2.M1, 5.3.2.1]; 5.3.2.1 [EC: 4.1.1.68, 5.3.2.M1, 5.3.2.1]

Predicted SEED Role

"small molecule metabolism; degradation; carbon compounds"

MetaCyc Pathways

• 4-hydroxyphenylacetate degradation (6/8 steps found)
• carbazole degradation (1/5 steps found)
• fluorene degradation I (1/8 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Fluorene degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.68, 5.3.2.-

Use Curated BLAST to search for 4.1.1.68 or 5.3.2.- or 5.3.2.1 or 5.3.2.M1

Sequence Analysis Tools

See A0A2Z5MH96 at UniProt or InterPro

Protein Sequence (63 amino acids)

>H281DRAFT_05328 4-oxalocrotonate tautomerase (Paraburkholderia bryophila 376MFSha3.1)
MPTFRIELFEGRSVEQKRQFVEAITKATCESLGVEPNSVDIILTDVKRENWATGGRLWSD
SGS