Protein Info for H281DRAFT_05313 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Polygalacturonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 PF00295: Glyco_hydro_28" amino acids 338 to 499 (162 residues), 59.3 bits, see alignment E=1.8e-20

Best Hits

KEGG orthology group: None (inferred from 94% identity to bug:BC1001_5592)

Predicted SEED Role

"Polygalacturonase (EC 3.2.1.15)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.2.1.15)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MHE9 at UniProt or InterPro

Protein Sequence (667 amino acids)

>H281DRAFT_05313 Polygalacturonase (Paraburkholderia bryophila 376MFSha3.1)
VATQYKSGSKGATSANSGRADPASPGRRAFMVFAGTSAGATLVGGLSACGGSGSSGLTPE
DPIWGSAGAATQIIASLAGVTQSMFPSRDFVVTQYGAQPCAVVAATNPYTGSSSPASPGF
NQTHAPSSFDSRPAFLAAIQACNAAGGGRVVVPSGTWYCAGPIVLLSNVNFHLSANCTIY
FSPNPADYAKDGPVDCGANGKLFYSRWQANDCLNYGSPVYARNQTNIALTGEDATSVLNG
QAMTPFSGSGNTATCWWTFKGSNGAYGCVSSSTPSQVYRNPNNVDLRTAAPGISDTLYAQ
LTSPTTPWQQDQNYLPALSEAGVPIAKRIFGIGHYLRPCMVEFIGCTNVLMENYCTNNTP
FWQHHPTACKNVVIRGVTTNSIGPNNDGFDPDACNNVLCDNVTFNTGDDCIAIKSGKDLD
TQYGPAQNHVIQNCTMNSGHGGITLGSEMGGGVQNIYARNLQMLNQNWATNPLNIAIRIK
TNMNRGGFVKNFYVDKVTLPNGVALKGGGYGSALLAGSPINGTVPLGVVTASAANPSAAQ
GGLITFDCDYQPANDAIRTRPAVVQNVNISNVQASNVTSGGVTGSCFQAIVAQGPVAFDY
NGPTPAPTIPPITGVTISNCNLGTAVCAGPATSTTPGPIYAYNVNGITLKNVVIGSTTYN
TNVTDLR