Protein Info for H281DRAFT_05282 in Paraburkholderia bryophila 376MFSha3.1

Annotation: malonate decarboxylase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 553 PF16957: Mal_decarbox_Al" amino acids 13 to 553 (541 residues), 988.1 bits, see alignment E=4.9e-302 TIGR01110: malonate decarboxylase, alpha subunit" amino acids 14 to 553 (540 residues), 938.9 bits, see alignment E=4.2e-287

Best Hits

Swiss-Prot: 59% identical to MADA_MALRU: Acetyl-S-ACP:malonate ACP transferase (madA) from Malonomonas rubra

KEGG orthology group: K13929, malonate decarboxylase alpha subunit [EC: 2.3.1.187] (inferred from 98% identity to bug:BC1001_5561)

MetaCyc: 68% identical to malonate decarboxylase acetyl-[acp]:malonate-[acp] transferase component (Klebsiella pneumoniae)
Biotin-independent malonate decarboxylase. [EC: 4.1.1.88]; Acetyl-S-ACP:malonate ACP transferase. [EC: 4.1.1.88, 2.3.1.187]

Predicted SEED Role

"Malonate decarboxylase alpha subunit" in subsystem Malonate decarboxylase

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.88

Use Curated BLAST to search for 2.3.1.187 or 4.1.1.88

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MGX8 at UniProt or InterPro

Protein Sequence (553 amino acids)

>H281DRAFT_05282 malonate decarboxylase alpha subunit (Paraburkholderia bryophila 376MFSha3.1)
MNPSAEASSAARSWTTRRDEKNRRLAVIAPWLEDGVLPPNRIVDALETLIQPGDRVALEG
DNQKQADFLSRSLAKVDPQKLHDVHLLISSISRPEHLALFERGIAHKVDFSFAGPQSLRV
AQLLEDGQLEIGAIYTYVELYARMFVDLTPHVALLCAEKADRHGNLYTGPNTEDTPTIAE
AAAFRHGIVIVQVNEIVDELPRVDIPGSWVDVVVQADRPFAVEPLFTRDPRHIGDLQVLT
AMMVIRGIYEPYGVSSLNHGIGFDTAAIELLLPTYGESLGLKGKICRNWTLNPHPTMIPA
IESGWVDSIHCFGSEVGMEAYIEARPDVFFTGNDGTLRSNRVLCQLAGQYGVDLFIGSTL
QIDADANSSTVTRGRLAGFGGAPNMGHDPRGRRHSSEAWLKLLKDQGPVSRGQKLVVQLA
ETYKKGGEPTFVDELDAVAVGAKNGMPIAPVMIYGDDVSHVVTEEGIAHLHKAEGIDERR
AAIAAVAGVTPIGLRAKPEKTAELRRRGIVAYPEDLGIRRGEAKRSLLAARSIDDLVTWS
GGLYAPPARFRSW