Protein Info for H281DRAFT_05281 in Paraburkholderia bryophila 376MFSha3.1

Annotation: triphosphoribosyl-dephospho-CoA synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 TIGR03132: triphosphoribosyl-dephospho-CoA synthase MdcB" amino acids 46 to 312 (267 residues), 314.4 bits, see alignment E=3.4e-98 PF01874: CitG" amino acids 48 to 310 (263 residues), 234.9 bits, see alignment E=7e-74

Best Hits

Swiss-Prot: 59% identical to MDCB_PSEAB: Probable 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (mdcB) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K13930, triphosphoribosyl-dephospho-CoA synthase [EC: 2.7.8.25] (inferred from 86% identity to bug:BC1001_5560)

MetaCyc: 55% identical to 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (Klebsiella pneumoniae)
2.7.8.25-RXN [EC: 2.4.2.52]

Predicted SEED Role

"Triphosphoribosyl-dephospho-CoA synthetase (EC 2.7.8.25)" in subsystem Malonate decarboxylase (EC 2.7.8.25)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.7.8.25

Use Curated BLAST to search for 2.4.2.52 or 2.7.8.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (320 amino acids)

>H281DRAFT_05281 triphosphoribosyl-dephospho-CoA synthase (Paraburkholderia bryophila 376MFSha3.1)
MRAAAVLFERDERSPAQAAQAVQAGPAQLPAARVSKFFQNLLTDAELAHYAVTALIDEAE
LTPKPALVDRRGSGAHRDLDLAIMRRSAQALEPTFDALARTARRRGEPSALLRTELAQIG
RAGEQDMLRATGGSNAHRGAIWIVGLLVAGASLQQTAGRLDASNICQRAAQIACFPDRFA
AATDSHGERVRQRYQVGGARREAQEGFPHVIGTGLPALIAARERGIAEDAARVDALLSIM
ASLDDTCLLHRAGLDGLHAGQRGAQRVLQAGGSSTREGQIALAALEQQLLSLNASPGGAA
DLLAATLFLDMLAHHDASGS