Protein Info for H281DRAFT_05126 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 4-cresol dehydrogenase (hydroxylating) (EC 1.17.99.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 PF01565: FAD_binding_4" amino acids 55 to 193 (139 residues), 111.4 bits, see alignment E=3e-36 PF02913: FAD-oxidase_C" amino acids 400 to 510 (111 residues), 35.9 bits, see alignment E=6.9e-13

Best Hits

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 91% identity to bxe:Bxe_A2781)

Predicted SEED Role

"4-cresol dehydrogenase [hydroxylating] flavoprotein subunit (EC 1.17.99.1)" in subsystem Cresol degradation (EC 1.17.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.3.15 or 1.17.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MBR3 at UniProt or InterPro

Protein Sequence (525 amino acids)

>H281DRAFT_05126 4-cresol dehydrogenase (hydroxylating) (EC 1.17.99.1) (Paraburkholderia bryophila 376MFSha3.1)
LIRHLPPGVSAAQFDRALAAWRSIVGEPHVVDSAAALSAYLDPFAPGEREAFAASAALLP
ASVDEIRAVLRVANQYRIPLWTVSTGRNFAYGGAAPRLTGSVVLDLQRMNRIVEVNETLA
YALVEPGVSYFDLHAHLRDKGYRLWVDPPAAGWGSVVGNTLERGFGYTPYGDHAATQCGM
EVVLANGDVLRTGMGAIDTSTAWQLYQPGYGPSFDAMFMQSNYGIVTKLGVWLMPAPPAY
LLGEIQFRDEADLETIVDILRPLRLDETIRNHAVIEGGLRRAAGLSARAQWYDGPGAMPE
SAVAAMLDKLDVGRWNLHFALYGTPEVVDAHYAIVQRAFARVPHARLLAKRYAGDAPPTA
GGDRNLAGIPAMSAFRMLDWRGGAGAHVDFAPVCPATGRDALRQYSMVKARAAEYGFDYY
GGFTAGVRHLHHIFAAIFDRDDTNQVEQAGALLRSLMSDARAAGYGQYRTHLAYMDFAAA
QYNFNDGALLRLSETIKDALDPNGVLAPGKQGIWPAAWRDRRGYT