Protein Info for H281DRAFT_05108 in Paraburkholderia bryophila 376MFSha3.1

Annotation: membrane fusion protein, multidrug efflux system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 418 transmembrane" amino acids 32 to 52 (21 residues), see Phobius details TIGR00998: efflux pump membrane protein" amino acids 28 to 353 (326 residues), 391.1 bits, see alignment E=1.9e-121 PF16576: HlyD_D23" amino acids 68 to 297 (230 residues), 48.7 bits, see alignment E=1.5e-16 PF13533: Biotin_lipoyl_2" amino acids 69 to 117 (49 residues), 36.5 bits, see alignment 8.3e-13 PF00529: CusB_dom_1" amino acids 185 to 353 (169 residues), 43.4 bits, see alignment E=6.9e-15 PF13437: HlyD_3" amino acids 226 to 337 (112 residues), 53 bits, see alignment E=1.2e-17

Best Hits

Swiss-Prot: 51% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 96% identity to bug:BC1001_1386)

MetaCyc: 51% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MCP4 at UniProt or InterPro

Protein Sequence (418 amino acids)

>H281DRAFT_05108 membrane fusion protein, multidrug efflux system (Paraburkholderia bryophila 376MFSha3.1)
MSTPQQPAANNQPAQPNKPAQPANSGKRKRMMTLLVIVILIAAIAYGLYYFLVARFHEDT
DDAYVNGNVVQITPQVTGTVVAVNADDTQTVKAGDPVVVLDPADARVALDQAEANLAQTV
RQVRGLFADDNQYQAQVAQRQSDLSRAQDDLKRRLTVAQTGAVSQEEISHARDAVKSAQA
AVEAAQQQLASNRALTANTTIANHPNVQASAAKVRDAYLNNARNTLPAPVTGYVAKRSVQ
VGQRVSPGTPLMAIVPLGAVWVDANFKEVQLKHMRIGQPVELTADVYGSSVVYHGKVVGF
SAGTGSAFSLLPAQNATGNWIKVVQRLPVRIALDPQELEKHPLRIGLSMQADVSIKDENG
GQLGNAQNTVYQTDVFAKYGDEADAEIARIIAENAGPNAGTHKAAQSGSSKPASAKLM