Protein Info for H281DRAFT_04847 in Paraburkholderia bryophila 376MFSha3.1

Annotation: mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)/mannose-6-phosphate isomerase, type 2 (EC 5.3.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 6 to 473 (468 residues), 746.3 bits, see alignment E=6.9e-229 PF00483: NTP_transferase" amino acids 8 to 289 (282 residues), 217.2 bits, see alignment E=5.5e-68 PF12804: NTP_transf_3" amino acids 9 to 145 (137 residues), 37.1 bits, see alignment E=7.4e-13 PF01050: MannoseP_isomer" amino acids 319 to 469 (151 residues), 252.1 bits, see alignment E=3.6e-79 PF07883: Cupin_2" amino acids 386 to 453 (68 residues), 43.4 bits, see alignment E=4.5e-15

Best Hits

Swiss-Prot: 61% identical to XANB_XANCP: Xanthan biosynthesis protein XanB (xanB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 84% identity to bph:Bphy_2293)

MetaCyc: 61% identical to mannose-1-phosphate guanylyltransferase (Escherichia coli K-12 substr. MG1655)
Mannose-1-phosphate guanylyltransferase. [EC: 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (477 amino acids)

>H281DRAFT_04847 mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)/mannose-6-phosphate isomerase, type 2 (EC 5.3.1.8) (Paraburkholderia bryophila 376MFSha3.1)
MANLPIVPVILAGGSGTRLWPLSRALFPKQFLALTGERTMLQETLARLAGISGLGSPMVI
CQDDHRFIVAEQLREHTSRDATIILEPVGRNTAPAIAIAALQALKDHPDPLLLVLAADHV
IADVDAFHRAVDTAAHSAETGALTTFGIEPVAPETGYGYLRAQPGEQGQVKRLATFVEKP
DLATAQAYLESGEYFWNSGMFLFRASVYLNELERYDPDMLKACVESLDRATKDLDFVRLD
KEAFTQCPENSIDYAVMEKTDKAVIVALNAGWNDVGSWSALWDVADKDDDGNVTKGDVIE
LGCENCFLQSSGGRLVAAVGLDNLIVVDTDDAVLIANKDDVQHVKQIVEKLRQDGRSEAV
QHRLVYRPWGSYDCIDRGSRYQVKRITVNPGARLSVQMHHHRAEHWVVVSGTARVKNGEN
ETLLTENESIYIPVGNIHALENPGKIPLELIEVQSGAYLGEDDIIRFDDRYGRHERT