Protein Info for H281DRAFT_04741 in Paraburkholderia bryophila 376MFSha3.1

Annotation: homogentisate 1,2-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR01015: homogentisate 1,2-dioxygenase" amino acids 20 to 443 (424 residues), 673.3 bits, see alignment E=6.7e-207 PF20510: HgmA_N" amino acids 21 to 289 (269 residues), 417.2 bits, see alignment E=2.9e-129 PF04209: HgmA_C" amino acids 290 to 443 (154 residues), 246.6 bits, see alignment E=8.2e-78

Best Hits

Swiss-Prot: 88% identical to HGD_BURP0: Homogentisate 1,2-dioxygenase (hmgA) from Burkholderia pseudomallei (strain 1106a)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 95% identity to bxe:Bxe_A3900)

MetaCyc: 53% identical to Homogentisate 1,2-dioxygenase (Homo sapiens)
Homogentisate 1,2-dioxygenase. [EC: 1.13.11.5]

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M8P2 at UniProt or InterPro

Protein Sequence (448 amino acids)

>H281DRAFT_04741 homogentisate 1,2-dioxygenase (Paraburkholderia bryophila 376MFSha3.1)
MDTSTRQPNTVRSRLEIEPGYQSGFGNEFATEALPGALPEGRNSPQRAAYGLYAEQLSGT
AFTAPRGHNRRTWLYRIRPAAVHKPFTPLPSERLVANFAEVPPTPPNQLRWDALPMPGEP
TDFVDGWVTMAGNGAADSMSGCAIHLYAANRSMTDRFFYSADGELLIVPQEGRLDIATEL
GRLEVRPFEIAVIPRGVRFAVSLPDGTARGYICENFGALLRLPDLGPIGSNGLANPRDFL
TPHAAYEDREGDFELVAKMNGNLWRADIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISF
DHPDPSIFLVLQSQSDTPGVDSLDFVIFPPRWLAAEDTFRPPWFHRNVASEFMGLVHGVY
DAKAEGFVPGGASLHNCMSGHGPDADTFEKASHSDTSKPVKVGDTMAFMFETRTLIKPTR
FALETAQLQAHYYECWQGLKKHFNPEQR