Protein Info for H281DRAFT_04700 in Paraburkholderia bryophila 376MFSha3.1

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 652 TIGR02350: chaperone protein DnaK" amino acids 3 to 606 (604 residues), 955.2 bits, see alignment E=6.3e-292 PF00012: HSP70" amino acids 4 to 606 (603 residues), 883.6 bits, see alignment E=5.7e-270 PF06723: MreB_Mbl" amino acids 114 to 378 (265 residues), 43.3 bits, see alignment E=2.2e-15

Best Hits

Swiss-Prot: 96% identical to DNAK_PARXL: Chaperone protein DnaK (dnaK) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 98% identity to bug:BC1001_0452)

MetaCyc: 73% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MA35 at UniProt or InterPro

Protein Sequence (652 amino acids)

>H281DRAFT_04700 molecular chaperone DnaK (Paraburkholderia bryophila 376MFSha3.1)
MGKIIGIDLGTTNSCVAIMEGNSVKVIENSEGARTTPSIIAYMEDGEILVGAPAKRQSVT
NPKNTLYAVKRLIGRRFEEKEVQKDIGLMPYKIMKADNGDAWVEVRDQKLAPPQISAEVL
RKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAALAFG
LDKNEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRIIDY
IIGEFKKEQGVDLSKDVLALQRLKESAEKAKIELSSSQQTEINLPYITADASGPKHLNLK
ITRAKLEALVEELIERTIEPCRVAIKDAGVKVGEIDDVILVGGMTRMPKVQEKVKEFFGK
DPRRDVNPDEAVAVGAAIQGQVLSGDRKDVLLLDVTPLSLGIETLGGVMTKMINKNTTIP
TKHSQVYSTADDNQSAVTIKVYQGEREMAAGNKLLGEFNLEGIPPAPRGTPQIEVSFDID
ANGILHVGAKDKATGKENRITIKANSGLSEAEIEKMVKDAEANAEEDHKLRELADARNQG
DALVHSTKKALTEYGDKLEAGEKEKIETALKDLEDALKSGSTDKAALEAKIEAVATASQK
MGEKMYADMQAAQGAEAAAAGAAGASAGPSAGGASQQQDDVVDADFKEVKKD