Protein Info for H281DRAFT_04682 in Paraburkholderia bryophila 376MFSha3.1
Annotation: glycolate oxidase FAD binding subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to GLCE_ECOLI: Glycolate oxidase subunit GlcE (glcE) from Escherichia coli (strain K12)
KEGG orthology group: K11472, glycolate oxidase FAD binding subunit (inferred from 97% identity to bgf:BC1003_0446)MetaCyc: 47% identical to glycolate dehydrogenase, putative FAD-binding subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]
Predicted SEED Role
"Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)
MetaCyc Pathways
- glycolate and glyoxylate degradation II (2/2 steps found)
- glycolate and glyoxylate degradation I (3/4 steps found)
- superpathway of glycol metabolism and degradation (5/7 steps found)
- photorespiration III (6/9 steps found)
- glycolate and glyoxylate degradation III (1/3 steps found)
- photorespiration II (6/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.14
Use Curated BLAST to search for 1.1.99.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5M7U8 at UniProt or InterPro
Protein Sequence (362 amino acids)
>H281DRAFT_04682 glycolate oxidase FAD binding subunit (Paraburkholderia bryophila 376MFSha3.1) MEEDDIVAMWSERVRSASAAGRALRVRGGGTKDWYGQTLEGEILDTRAYRGIIAYDPAEL VITARAGTPLLEIEAALAEHHQMLAFEPPHFGPQATFGGCIAAGIAGPRRPSAGAARDFV LGAVVMNGQGQALHFGGQVVKNVAGYDVSRLMAGSLGTLGLILELSVKVLPQPQAEATLK FDVNGTDAVRKLNEWGGRPLPITASAWRHGTLAVRLAGAESAVKSARASLGGEVVDAVEA ERFWAGLREQTDTFFAAIPPKAALWRLALPSITEPLQLPGAQLMEWGGAQRWWITDTDAQ TVRISAKQAGGHATIFRTGHGYDRGAGVFTPLPAPLMKIHRGLKTAFDPARIFNRGRLYP DF