Protein Info for H281DRAFT_04672 in Paraburkholderia bryophila 376MFSha3.1

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 PF17191: RecG_wedge" amino acids 117 to 263 (147 residues), 38.5 bits, see alignment E=2.3e-13 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 127 to 768 (642 residues), 712.1 bits, see alignment E=3.8e-218 PF04851: ResIII" amino acids 387 to 534 (148 residues), 33.5 bits, see alignment E=9.6e-12 PF00270: DEAD" amino acids 392 to 540 (149 residues), 86.3 bits, see alignment E=5.2e-28 PF00271: Helicase_C" amino acids 586 to 690 (105 residues), 58.1 bits, see alignment E=2.6e-19 PF19833: RecG_dom3_C" amino acids 719 to 781 (63 residues), 41 bits, see alignment 4.4e-14

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 94% identity to bpy:Bphyt_0697)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (796 amino acids)

>H281DRAFT_04672 ATP-dependent DNA helicase RecG (Paraburkholderia bryophila 376MFSha3.1)
MPLSDRRLPSATDEVSAEPAAPVARGGTAADASGQADGAAQPGRTARAVKRADQTSGDEP
DEASRRTSQQARQQTPDGMTAPAPQAAAGKARASAKSAAKSSAKSSAKPAAKPVDKLAKL
GLTRDIDLVLHLPMRYEDETSLTPIGHLLPGGIAQTEGVVFDNEIAYRPRRQLLVKLRDD
AGDELVLRFLNFYGSQVKQMAIGARLRVRGDVRGGFFGMEMVHPVVRVVDEDTPLPQALT
PVYPSTAGVTQAYLRKSIDNALSRTSLPELLPEAVARQWLQPLGVPSLMDAVRTLHHPGA
QSDETALIDGTHPAWVRIKFEELLAQQMSLKRAHEERRTRSAPPMPRRKLGDESALVARL
LKALPFSLTRAQERVGGEIALDLTQPHPMQRLLQGDVGSGKTIVAALAAAQAIDAGYQAA
LMAPTEILAEQHARKLRGWLEPLGVNVAWLAGSLKAKEKRAAIEAAALGTAQLVIGTHAI
IQDAVEFARLGLVIVDEQHRFGVAQRLALRAKAKNAADGARDFQPHQLMMSATPIPRTLA
MTYYADLDVSTIDELPPGRTPILTKLVSDARREEVIGRVREAALTGRQVYWVCPLIEESE
TLQLQTAVETYETLVAALPELNVGLVHGRLAPAEKAAVMDAFTRNEVQLLVATTVIEVGV
DVPNASLMVIEHAERFGLAQLHQLRGRVGRGSAASVCVLLYTGPLSMTARARLQTMRETT
DGFEIARRDLEIRGPGEFLGARQSGAAMLRFADLQNDQWLIEPAREAAAALLETYPDVVT
QHLARWLGAREQYLKA