Protein Info for H281DRAFT_04671 in Paraburkholderia bryophila 376MFSha3.1

Annotation: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 transmembrane" amino acids 311 to 328 (18 residues), see Phobius details TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 2 to 347 (346 residues), 413.1 bits, see alignment E=4e-128 PF02547: Queuosine_synth" amino acids 4 to 347 (344 residues), 425.3 bits, see alignment E=7.8e-132

Best Hits

Swiss-Prot: 95% identical to QUEA_PARXL: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 94% identity to bug:BC1001_0421)

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-

Use Curated BLAST to search for 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M8J6 at UniProt or InterPro

Protein Sequence (355 amino acids)

>H281DRAFT_04671 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (Paraburkholderia bryophila 376MFSha3.1)
MFKLSDFDFDLPPELIAQVALPERSASRLLQVDNAAHADGAARLVDRRFAELPECISAGD
LLVFNDTKVLKARFLGQKASGGKIEVLVERLTGERTALAQIRASKSPQQGTTIRLADAFD
VTVGERVEPFYTLHFPDDCLTLIEQFGRLPLPPYIEHDPDSTDETRYQTVFAQNPGAVAA
PTAGLHFDDALLARLDTAGVERATLTLHVGAGTFQPVRVENLAEHKMHSEWYHLPQSLAD
RIAATKARGNRVIAVGTTSMRALEAAARDAEAAGRPLAAASTETDIFITPGYRFRVVDRL
VTNFHLPKSTLLMLVSAFAGVETIRAAYRHAIEERYRFFSYGDAMLLTRRDDRAV