Protein Info for H281DRAFT_04649 in Paraburkholderia bryophila 376MFSha3.1

Annotation: glycyl-tRNA synthetase alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 3 to 300 (298 residues), 514.6 bits, see alignment E=3.7e-159 PF02091: tRNA-synt_2e" amino acids 4 to 293 (290 residues), 487.4 bits, see alignment E=6e-151

Best Hits

Swiss-Prot: 82% identical to SYGA_BORA1: Glycine--tRNA ligase alpha subunit (glyQ) from Bordetella avium (strain 197N)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 97% identity to bph:Bphy_0406)

MetaCyc: 72% identical to glycine--tRNA ligase subunit alpha (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M7S6 at UniProt or InterPro

Protein Sequence (335 amino acids)

>H281DRAFT_04649 glycyl-tRNA synthetase alpha chain (Paraburkholderia bryophila 376MFSha3.1)
MLTFQQIILTLQSYWDKQGCALLQPIDMEVGAGTSHVHTFLRAVGPEPWRAAYVQPSRRP
KDGRYGENPNRLQHYYQYQVVLKPAPENILDLYLGSLEALGFDLKQNDVRFVEDDWENPT
LGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCKPVLGEITYGLERLAMYLQKVENVYDLVW
TEWEEQGPNGPETRRLTYGDVYHQNEVEQSTYNFEHANVDLLFTFFNSYEAEAKRMIEAQ
IALPAYELVLKAGHTFNLLDARGAISVTERAAYIGRIRALSRLVAQAYYDSREKLGFPML
GNPVHAVPGLTTDEQDAAMPAWAPPLKVERKIDPD