Protein Info for H281DRAFT_04648 in Paraburkholderia bryophila 376MFSha3.1

Annotation: glycyl-tRNA synthetase beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 5 to 698 (694 residues), 668.5 bits, see alignment E=8.1e-205 PF02092: tRNA_synt_2f" amino acids 9 to 560 (552 residues), 628.5 bits, see alignment E=1.2e-192 PF05746: DALR_1" amino acids 590 to 689 (100 residues), 41.3 bits, see alignment E=1.5e-14

Best Hits

Swiss-Prot: 66% identical to SYGB_RALPJ: Glycine--tRNA ligase beta subunit (glyS) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 96% identity to bxe:Bxe_A4013)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M7P3 at UniProt or InterPro

Protein Sequence (700 amino acids)

>H281DRAFT_04648 glycyl-tRNA synthetase beta chain (Paraburkholderia bryophila 376MFSha3.1)
MTQSHQATLLVELLTEELPPKALARLGDAFAEGIAERLAARDLIEGELSFERYATPRRLA
VTIKNVRTVAPEKHVREKVLPVSVALDKDGQPTAPLAKKLAALGFPDFSVNDLERAQDGK
AEAFFLRYAAPGATLAEGLQAALDETLAKLPIPKVMTYQRPDGTNVQFVRPAHRLTALHG
EQIVPVSALGIDADDTTLGHRFLSEGFVQIQHADSYADTLMHKGRVVPNFADRKETIRTH
LLAHADGDQVVMPESLLDEVTSLVEWPVVYACRFEDEFLQVPQECLILTMQTNQKYFALT
DENGKLRSRFLIVSNIETSTPGDIVEGNERVVRPRLADAKFFFEQDKKKPLADRVPLLAN
VVYHNKLGSALQRVERVEALAGAIAGLIGADVALAKRAARLAKADLITDMVGEFPELQGT
MGTYYARHDGEPEEVALACSEHYQPRFSGDALPGTATGTVVALADKLETLVGIWGIGLQP
TGEKDPFALRRHALGVLRILVEKQLAVDFVELLRTAYAQFAAVPNVVDSTQAIYDFSMDR
LRGLLRERGHAPGEIDAVLALNPTRLDDIVARLDAVREFAALPEAASLAAANKRISNILK
KSEGATATGGVQVTLLFEPAEKALHAQLEQVAPRVQSQLAARDYTGALTALAALREPVDT
FFNDVMVNAEDPALRANRLALLGALHQQMNCVADISRLAA