Protein Info for H281DRAFT_04643 in Paraburkholderia bryophila 376MFSha3.1

Annotation: methylmalonyl-CoA epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 128 PF00903: Glyoxalase" amino acids 2 to 123 (122 residues), 108.5 bits, see alignment E=4.2e-35 TIGR00068: lactoylglutathione lyase" amino acids 2 to 128 (127 residues), 214 bits, see alignment E=3.4e-68 PF13669: Glyoxalase_4" amino acids 5 to 107 (103 residues), 44.1 bits, see alignment E=3.6e-15 PF18029: Glyoxalase_6" amino acids 7 to 121 (115 residues), 31.7 bits, see alignment E=3.1e-11

Best Hits

Swiss-Prot: 78% identical to LGUL_NEIMB: Lactoylglutathione lyase (gloA) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K01759, lactoylglutathione lyase [EC: 4.4.1.5] (inferred from 94% identity to bug:BC1001_0393)

MetaCyc: 64% identical to lactoylglutathione lyase (Escherichia coli K-12 substr. MG1655)
Lactoylglutathione lyase. [EC: 4.4.1.5]

Predicted SEED Role

"Lactoylglutathione lyase (EC 4.4.1.5)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 4.4.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M7N1 at UniProt or InterPro

Protein Sequence (128 amino acids)

>H281DRAFT_04643 methylmalonyl-CoA epimerase (Paraburkholderia bryophila 376MFSha3.1)
MRLLHTMLRVGDLDRSIAFYTELLGMKLLRREDYPDGKFTLAFVGYTDERDGTVIELTHN
WDTPAYDLGNGFGHLAVEVEDAYAACDKIKAQGGTVVREAGPMKHGTTVIAFVTDPDGYK
IEFIQKKK