Protein Info for H281DRAFT_04620 in Paraburkholderia bryophila 376MFSha3.1

Annotation: aspartyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 TIGR00459: aspartate--tRNA ligase" amino acids 3 to 591 (589 residues), 823.5 bits, see alignment E=4.4e-252 PF01336: tRNA_anti-codon" amino acids 20 to 103 (84 residues), 66.3 bits, see alignment E=2.9e-22 PF00152: tRNA-synt_2" amino acids 121 to 567 (447 residues), 401.7 bits, see alignment E=3.4e-124 PF02938: GAD" amino acids 312 to 410 (99 residues), 91.9 bits, see alignment E=3.7e-30

Best Hits

Swiss-Prot: 98% identical to SYDND_PARXL: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 98% identity to bgf:BC1003_0382)

MetaCyc: 58% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5M9W6 at UniProt or InterPro

Protein Sequence (599 amino acids)

>H281DRAFT_04620 aspartyl-tRNA synthetase (Paraburkholderia bryophila 376MFSha3.1)
MSMRSEYCGLVTEELLGQTVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFK
AAEGVRNEFCLQIKGVVRNRPEGTTNAALKSGKIEVLCHELIVLNASITPPFQLDDDNLS
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDAQGFVDIETPMLTKSTPEGARDYL
VPSRTNPGQFFALPQSPQLFKQLLMVANFDRYYQIVKCFRDEDLRADRQPEFTQIDCETS
FLSEQEIRDLFEAMIRHVFKETIGVELDEKFPVMQYSEAMRRFGSDKPDLRVKLEFTDLT
DAVKDVDFKVFSTPANTKDGRVAAIRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKVNE
VAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIFFAADRAKVVNDSLGALRLKIGHS
EFGKANGLVESGWKPLWVVDFPMFEYDEEDNRYVAAHHPFTSPKDEHLEYLETDPARCLA
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKINAEEAQAKFGFLLDALQYGAPPHGGI
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSEVDERQLRELHIRLRTPEPKA