Protein Info for H281DRAFT_04595 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3-hydroxyacyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03446: NAD_binding_2
  • amino acids 10 to 125 (116 residues), 22.1 bits, E=2e-08
• PF02737: 3HCDH_N
  • amino acids 10 to 188 (179 residues), 199.9 bits, E=5.3e-63
• PF00725: 3HCDH
  • amino acids 191 to 288 (98 residues), 85.5 bits, E=4.4e-28
  • amino acids 418 to 501 (84 residues), 73.1 bits, E=3.3e-24

Best Hits

KEGG orthology group: K00074, 3-hydroxybutyryl-CoA dehydrogenase [EC: 1.1.1.157] (inferred from 93% identity to bug:BC1001_1112)

MetaCyc: 67% identical to 3-hydroxyacyl-CoA dehydrogenase (Cupriavidus necator H16)
3-hydroxyacyl-CoA dehydrogenase. [EC: 1.1.1.35]

Predicted SEED Role

"3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 1.1.1.35, EC 4.2.1.17)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• phenylacetate degradation I (aerobic) (9/9 steps found)
• adipate degradation (5/5 steps found)
• superpathway of phenylethylamine degradation (9/11 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• benzoyl-CoA degradation I (aerobic) (5/7 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• valproate β-oxidation (6/9 steps found)
• glycerol degradation to butanol (11/16 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• 2-methylpropene degradation (2/8 steps found)
• L-tryptophan degradation III (eukaryotic) (7/15 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (3/17 steps found)
• androstenedione degradation I (aerobic) (8/25 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• superpathway of testosterone and androsterone degradation (8/28 steps found)
• platensimycin biosynthesis (6/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• androstenedione degradation II (anaerobic) (6/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (11/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (11/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (7/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.157, 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.157 or 1.1.1.35 or 4.2.1.17

Sequence Analysis Tools

See A0A2Z5MFM3 at UniProt or InterPro

Protein Sequence (521 amino acids)

>H281DRAFT_04595 3-hydroxyacyl-CoA dehydrogenase (Paraburkholderia bryophila 376MFSha3.1)
MTSRNFSIETIGIVGTGAMGRGIAQIAALAGLTVRLYDANPAAIGAARDYLAETFAKLTA
KGKLEQARSLAALANVTAAHAITDLADCDLVIEAIVEKLEVKQALFRELETVVSGRCVLA
SNTSSLSITAIAAGCTDPSRVAGYHFFNPVPLMKVVEVIDGLRSDRQAGDALMDLARRMG
HTPVRAKDMPGFIVNHAGRGMNTEGLRVAGEGVASFVDIDRIMREQAGFRLGPFELLDLT
ALDVSHPVMESIYHQFYEEPRFTPSPITGTRLAGGLLGRKTGEGFYRYEDGKQQAPAETP
APAALPTSVWVSKRYREAYQAVVELVGKAGVKLDDGASPAADSLIVVTPFGHDATTAAVD
EALDASRVVAIDALFPLVGTQRRTLMTTPATTRAARDAGHALFSADGVPVTVIRDSTGFV
AQRVVATIVNIGCDIAQKQIATPEDIDLAVMLGLGYPRGPLALGDALGAKTILTILRNMF
DVLGDPRYRPSPWLARRAQLGMSLTQSDAADTQTENPETQQ