Protein Info for H281DRAFT_04579 in Paraburkholderia bryophila 376MFSha3.1

Annotation: NADH dehydrogenase subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 35 to 55 (21 residues), see Phobius details amino acids 85 to 105 (21 residues), see Phobius details amino acids 113 to 132 (20 residues), see Phobius details amino acids 138 to 156 (19 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details amino acids 203 to 223 (21 residues), see Phobius details amino acids 241 to 260 (20 residues), see Phobius details amino acids 272 to 295 (24 residues), see Phobius details amino acids 302 to 322 (21 residues), see Phobius details amino acids 330 to 351 (22 residues), see Phobius details amino acids 372 to 393 (22 residues), see Phobius details amino acids 405 to 426 (22 residues), see Phobius details amino acids 452 to 469 (18 residues), see Phobius details TIGR01972: proton-translocating NADH-quinone oxidoreductase, chain M" amino acids 7 to 486 (480 residues), 628.2 bits, see alignment E=4.7e-193 PF00361: Proton_antipo_M" amino acids 133 to 415 (283 residues), 270.6 bits, see alignment E=7.8e-85

Best Hits

Swiss-Prot: 49% identical to NUOM_RICPR: NADH-quinone oxidoreductase subunit M (nuoM) from Rickettsia prowazekii (strain Madrid E)

KEGG orthology group: K00342, NADH dehydrogenase I subunit M [EC: 1.6.5.3] (inferred from 97% identity to bug:BC1001_1096)

MetaCyc: 42% identical to NADH-quinone oxidoreductase subunit M (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MEG4 at UniProt or InterPro

Protein Sequence (496 amino acids)

>H281DRAFT_04579 NADH dehydrogenase subunit M (Paraburkholderia bryophila 376MFSha3.1)
MHAYPILSIAIWLPILFGLLVLAIGSDRNPGPTRWVALIGSVASFIVTIPLITGFDSSSA
DLQFVEKANWIERFNITYHLGVDGISMWFVVLTALITVIVVIAAWEVITKNVGQYLASFL
ILSGIMVGVFSAADGMLFYVFFEATLIPMYIIIGVWGGANRVYAAFKFFLYTLMGSLLML
VALLYLYVQTGTFDLATWQHAQIAMTPQVLLFIAFFMAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSMPIAPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIVQMISHGFVSGAMFLSIGVLYDRMHSRQIA
DYGGVVNVMPKFAAFVMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAGGAAVTLILG
AAYTLWMYKRVYFGAVANDHVKSLVDISRREFFMLAVLAALTLFMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLSQ