Protein Info for H281DRAFT_04562 in Paraburkholderia bryophila 376MFSha3.1

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 715 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 12 to 698 (687 residues), 1064.2 bits, see alignment E=0 PF01138: RNase_PH" amino acids 17 to 146 (130 residues), 100.9 bits, see alignment E=1.9e-32 amino acids 331 to 463 (133 residues), 105.4 bits, see alignment E=7.9e-34 PF03725: RNase_PH_C" amino acids 149 to 212 (64 residues), 45.8 bits, see alignment E=1.2e-15 amino acids 468 to 536 (69 residues), 25.6 bits, see alignment E=2.5e-09 PF03726: PNPase" amino acids 244 to 327 (84 residues), 68.8 bits, see alignment E=1.1e-22 PF00013: KH_1" amino acids 563 to 620 (58 residues), 45.8 bits, see alignment 1e-15 PF00575: S1" amino acids 626 to 697 (72 residues), 63.9 bits, see alignment E=3.7e-21

Best Hits

Swiss-Prot: 97% identical to PNP_PARXL: Polyribonucleotide nucleotidyltransferase (pnp) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 98% identity to bug:BC1001_1080)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MFY4 at UniProt or InterPro

Protein Sequence (715 amino acids)

>H281DRAFT_04562 polyribonucleotide nucleotidyltransferase (Paraburkholderia bryophila 376MFSha3.1)
MTMFSKIVKEFKWGQHNVRLETGEIARQASGAVIVDVEDTVVLATVVGAKTAKPGQDFFP
LTVDYLEKTYAAGKIPGGFFRREGRPSEGETLISRLIDRPLRPLFPEGFYNEVQVVIHVL
SLNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYINNEYVLNPTRPQMKESALDLI
VAGTERAVLMVESEAQQLSEEVMLGGVVFGHEQMQIAIDAIHDLVREGGKPEWDWQPAAK
NEPLIARVTELAQAELLAAYQIRDKQARSTKLKEIYAAVSAKLEQDASANGTVAADKATV
GNVLFDIEAKIVRTQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
ATLGTKGDEQNIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALAA
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTEHGVTALQMDIKIQGITKEIMQVALAQAKEGRMHI
LSKMTSAVSGANTQLSEFAPRMITIKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
VVTIASTSSEGMAEAKKRIENITLEVEVGQVYEGTVLKLLDFGAIVNILPGKDGLLHISE
IANERIKDINDYLKDGQQVKVKVIQTDEKGRVRLSAKALLNDGAPSAPQGEPTPQ