Protein Info for H281DRAFT_04507 in Paraburkholderia bryophila 376MFSha3.1
Annotation: phenylalanyl-tRNA synthetase beta subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to SYFB_BURP1: Phenylalanine--tRNA ligase beta subunit (pheT) from Burkholderia pseudomallei (strain 1710b)
KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 97% identity to bgf:BC1003_2377)Predicted SEED Role
"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)
MetaCyc Pathways
- tRNA charging (20/21 steps found)
- ergotamine biosynthesis (4/12 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.20
Use Curated BLAST to search for 6.1.1.20
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A2Z5MES3 at UniProt or InterPro
Protein Sequence (811 amino acids)
>H281DRAFT_04507 phenylalanyl-tRNA synthetase beta subunit (Paraburkholderia bryophila 376MFSha3.1) MQFPESWLRTFVDPQLTTDELSHALTMAGLEVEDLRPAAPPTSKIVVGQVLEVVKHPDAD KLNVCQVNAGTGATLNIVCGAPNVAPGIKVPVALVGAQLPPAEEGGAPFEIKLSKLRGVE SQGMLCSARELKLSEDHSGLLILPEDTPIGQDIRETLNLDDTIFEIKLTPNKADCLSVFG VARETSAITGAPLRPLEIKPVQVKLDETLPVKISAPDLCGRFSGRVIRGVNARAKSPQWM VERLERSGQRSISALVDISNYVMLELGRPSHVFDLDKIHGGMDVRWGRKGETLKLLNGNT VEVDETVGVIADEQHIESLAGIMGGDSTAVTLDTTNIYLEAAFWWPDSIRGRSRRYNFST DAGHRFERGVDYATTVEHIERITQLILDICGGEAGPVDDQVVNVPQREPVKMRVSRANRI IGIAIGAEEIAQIFTRLGLTFEHEGDTFSVMPPSYRFDIEIEEDLIEEVARIYGFEKIPA RPPVAKSEMRATNETRRSIHAIRHALAARDYAETVNFSFVDAEWEQDFAGNDKPVRLLNP IASQLSVMRTTLIGSLIHVLRTNLNRRAADRVRVFEAGRVFLHDPSIKAGELTVEGFAQP KMIGGLAYGPALEEQWGAPSRTVDYFDVKADVEALLSPAVARFVKAEHPALHPGRSARIE LNGRAIGWIGELHPRWMQKYDLPNAPILFEVEADALMQRVLPTPSEVSKFPPVRRDIAVV VDQKIEAQALLDELQKAQSEPACKTVQKVVLFDEFRPKSNTSGGLGAHEKSLAFRVTLQD TGGTLQDETVDLAIQTLVERLARVYGARLRG