Protein Info for H281DRAFT_04486 in Paraburkholderia bryophila 376MFSha3.1

Annotation: O-acetylhomoserine sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase" amino acids 11 to 428 (418 residues), 576.7 bits, see alignment E=1.2e-177 PF01053: Cys_Met_Meta_PP" amino acids 13 to 429 (417 residues), 425.2 bits, see alignment E=3.7e-131

Best Hits

Swiss-Prot: 50% identical to FUB7_FUSO4: Sulfhydrylase FUB7 (FUB7) from Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 93% identity to bpy:Bphyt_2770)

MetaCyc: 46% identical to O-acetylhomoserine sulfhydrylase (Corynebacterium glutamicum ATCC 13032)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MCC2 at UniProt or InterPro

Protein Sequence (465 amino acids)

>H281DRAFT_04486 O-acetylhomoserine sulfhydrylase (Paraburkholderia bryophila 376MFSha3.1)
VASPTSMSANRFDTLALHAGAAPDPATGARATPIYQTTSFSFRDSDHAAALFNMERAGHV
YSRISNPTVAVFEERVAALENGAGAIGTASGQAALHLAIVTLMGRGSHIVASSALYGGSH
NLLHYTLRRFGIETTFVKPGDIDAWRAALRPNTRLLFGETLGNPGLDVLDVATVAQIAHE
HRVPLLVDSTFTTPYLLKPFEHGADFVYHSATKFLGGHGTTIGGVLVDGGTFDFDASGRF
PEFTEPYEGFHGIIFSEESTAAPFLLRARREGLRDFGACLHPQAAWQLLQGIETLPLRME
RHVANTRRVVEFLAGHAAVEAVAYPELPTHPDHALAKRLLPRGAGAVFSFNLRGDRAAGR
SFIEALSLFSHLANVGDARSLVIHPASTTHFRMDAAALAAAGIAEGTIRLSIGLEDPDDL
IDDLKRGLKAAQKASGSTAPRSGQSGSTEPGGSGSAATRPGKESA