Protein Info for H281DRAFT_04471 in Paraburkholderia bryophila 376MFSha3.1

Annotation: propionyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02316: propionate--CoA ligase
  • amino acids 3 to 629 (627 residues), 1102 bits, E=0
• PF16177: ACAS_N
  • amino acids 4 to 58 (55 residues), 87.8 bits, 4.9e-29
• PF00501: AMP-binding
  • amino acids 68 to 443 (376 residues), 252.1 bits, E=1.3e-78
• PF13193: AMP-binding_C
  • amino acids 511 to 594 (84 residues), 92.7 bits, E=3.3e-30

Best Hits

KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 95% identity to bgf:BC1003_2412)

Predicted SEED Role

"Propionate--CoA ligase (EC 6.2.1.17)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 6.2.1.17)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• β-alanine biosynthesis II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• acrylate degradation II (1/3 steps found)

KEGG Metabolic Maps

• Propanoate metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.17

Use Curated BLAST to search for 6.2.1.17

Protein Sequence (635 amino acids)

>H281DRAFT_04471 propionyl-CoA synthetase (Paraburkholderia bryophila 376MFSha3.1)
MTRYRDFHRRSIENPEAFWRDEARRIHWETPFDTVLDRSNPPFARWFVGGRTNLCHNAVD
RHLVDRAQQNALVYVSTETGIERRYTYAELYAEINRMAAVMRSLGVKRGDVVLLYLPMIP
EALFAMLACARLGAIHSVVFGGFAAPNLAARIDDAKPVLIVTADAGARGGKVIDYTPLVD
EAIARATHKTARVLLIDRQLAPERLNATYLVDYEPLREQFFDAHVSCEWLESSEPSYVLY
TSGTTGKPKGVQRDVGGYAVALAASMEYIFEGKAGDTMFTASDVGWVVGHSYIVYAPLIA
GLTTVMYEGTPIRPDGGIWWRLVEQHKINLMFTAPTALRVLKKQDPALLESADLSSLRTL
FLAGEPLDEPTASWITGALGKPVIDNYWQTETGWPMLAIPRGVEALPTKLGSPGVPSAGF
NLTLRNELTGEPCPPGEKGVLTLNYPLPPGCMSTVWGDDKRFISTYWSSFPDQQVYSTFD
WGIQDEDGYVTILGRTDDVINVAGHRLGTREIEEALSSHAAVAEVAVVGVADPLKGQAAM
AFVVLRDAQAYADVDARTKLEAELTATVDRQLGAIARPSRVVIVSMLPKTRSGKLLRRAI
AALAEGREPGELPTIEDPAALQQVREGLSESARNE