Protein Info for H281DRAFT_04458 in Paraburkholderia bryophila 376MFSha3.1

Annotation: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR04377: 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase
  • amino acids 26 to 637 (612 residues), 914.5 bits, E=1.8e-279
• PF02776: TPP_enzyme_N
  • amino acids 28 to 155 (128 residues), 68.4 bits, E=7.3e-23
• PF00205: TPP_enzyme_M
  • amino acids 242 to 374 (133 residues), 118.3 bits, E=3.2e-38
• PF02775: TPP_enzyme_C
  • amino acids 442 to 596 (155 residues), 117.3 bits, E=8.2e-38

Best Hits

KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 90% identity to bug:BC1001_2456)

Predicted SEED Role

"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)

MetaCyc Pathways

• myo-inositol degradation I (6/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (6/10 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Caprolactam degradation
• Ethylbenzene degradation
• Inositol phosphate metabolism
• Limonene and pinene degradation
• Nicotinate and nicotinamide metabolism
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.7.1.-

Use Curated BLAST to search for 3.7.1.-

Protein Sequence (661 amino acids)

>H281DRAFT_04458 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (Paraburkholderia bryophila 376MFSha3.1)
MNQRELQHGHDEAASAGAVVSGTTIRLTTAQALVRYLAAQRVATEDGSGTEPLFGGVFAI
FGHGNVAGMGEALYQHRDELPTLRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSIGPGATN
LLTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFHDGGVSANDAFKPVSRYFDRIM
HPAQLLSALPRALRVLTDAASCGPVTLALPQDVQAQAWDFPADFFAPRIVTFYAPAPRVD
EIDAAVARLQRAKRPLIVAGGGVLYGRATDALQRFAAAHGIPVAETQAGKSSLAWDDPLN
AGSLGVTGSPAANALARDADCVLALGTRLQDFTTGSNTLFTQADVIGINANAFDALKHRA
QVVEADARLALDALAERLQDWQADRAWTSRAHELAASWRDTVHTLTHAPQRESVLPYEGD
VIGAVQRSSTDSPANDIVVCAAGTLPGELHKLWRAGTPGAYHVEYGYSCMGYEIAGGLGV
KLARPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPF
NNLLEDSMQGPLGAPQIDFAAHARALGARAEHAANVAELEAALQRARSADRTYVISIDTD
PARTTSDGGWWWEVAVPEVSPRASVREARANYDAQISARGKPPASAENSGNGSNSNDQAN
D