Protein Info for H281DRAFT_04414 in Paraburkholderia bryophila 376MFSha3.1

Annotation: phosphate ABC transporter ATP-binding protein, PhoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 282 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 36 to 282 (247 residues), 396.2 bits, see alignment E=2.6e-123 PF00005: ABC_tran" amino acids 51 to 206 (156 residues), 125.1 bits, see alignment E=3.3e-40

Best Hits

Swiss-Prot: 97% identical to PSTB_PARXL: Phosphate import ATP-binding protein PstB (pstB) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 99% identity to bgf:BC1003_2465)

MetaCyc: 80% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.27

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MCZ2 at UniProt or InterPro

Protein Sequence (282 amino acids)

>H281DRAFT_04414 phosphate ABC transporter ATP-binding protein, PhoT family (Paraburkholderia bryophila 376MFSha3.1)
MNMAETQLNPISRPTAPAGFDPAQGGQEPSPSRPKIEVNDLNFFYGKYHALKNINLQIPE
GKVTAFIGPSGCGKSTLLRTFNKMYALYPEQRAEGEILMDGENLLTSKRDISLLRARIGM
VFQKPTPFPMSIYDNIAFGVKMFETLPRSEMDDRVEWALTKAALWNEVKDKLGQSGYGLS
GGQQQRLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELIAELKSDYTVVIVTHNMQQA
ARCSDYTAYMYLGELIEFGDTEKIFIKPVRKETEDYITGRFG