Protein Info for H281DRAFT_04399 in Paraburkholderia bryophila 376MFSha3.1

Annotation: hydroxyacylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 263 TIGR03413: hydroxyacylglutathione hydrolase" amino acids 7 to 262 (256 residues), 326.1 bits, see alignment E=6.8e-102 PF00753: Lactamase_B" amino acids 16 to 173 (158 residues), 59.7 bits, see alignment E=6.1e-20 PF12706: Lactamase_B_2" amino acids 27 to 89 (63 residues), 28.4 bits, see alignment E=1.9e-10 PF16123: HAGH_C" amino acids 175 to 262 (88 residues), 88 bits, see alignment E=6.5e-29

Best Hits

Swiss-Prot: 58% identical to GLO2_CUPMC: Hydroxyacylglutathione hydrolase (gloB) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K01069, hydroxyacylglutathione hydrolase [EC: 3.1.2.6] (inferred from 89% identity to bpy:Bphyt_2871)

Predicted SEED Role

"Hydroxyacylglutathione hydrolase (EC 3.1.2.6)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 3.1.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MF14 at UniProt or InterPro

Protein Sequence (263 amino acids)

>H281DRAFT_04399 hydroxyacylglutathione hydrolase (Paraburkholderia bryophila 376MFSha3.1)
MNALEYVPVPAFEDNYIWVVSDGYHAVVVDPGEAAPVRAYLARRGWRLSAILLTHHHQDH
VGGVADLLNGQAVPVYGPAGEAIGHLTHRLKHGDRVTIAAPALDLSVLEVPGHTSGHIAY
FQAADPGGTPHVFCGDTLFACGCGRLFEGTPSQMLASLDSLAALPGATEVHCAHEYTLSN
IRFALACDPDNDELLAWRDKASDLRARKVPTLPTTIAHERAVNPFLRADNPAIQTTLREQ
LHADVPDRLASFTLMREWKNRFR