Protein Info for H281DRAFT_04360 in Paraburkholderia bryophila 376MFSha3.1

Annotation: GTP-binding protein LepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 3 to 181 (179 residues), 183.7 bits, E=7.2e-58
• TIGR01393: elongation factor 4
  • amino acids 3 to 595 (593 residues), 996.9 bits, E=3e-304
• TIGR00231: small GTP-binding protein domain
  • amino acids 5 to 173 (169 residues), 61.5 bits, E=8.5e-21
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 43.1 bits, E=1.2e-14
• PF00679: EFG_C
  • amino acids 399 to 482 (84 residues), 80 bits, E=2.8e-26
• PF06421: LepA_C
  • amino acids 487 to 592 (106 residues), 167.8 bits, E=1.6e-53

Best Hits

Swiss-Prot: 98% identical to LEPA_PARXL: Elongation factor 4 (lepA) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 98% identity to bge:BC1002_2132)

MetaCyc: 70% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A2Z5ME18 at UniProt or InterPro

Protein Sequence (597 amino acids)

>H281DRAFT_04360 GTP-binding protein LepA (Paraburkholderia bryophila 376MFSha3.1)
MDHIRNFSIIAHIDHGKSTLADRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQTAA
LTYRARDGKVYNLNMIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
ELGVDVIPVLNKIDLPAANPENAIAEIEDVIGIDATDATHCSAKTGQGVEDVLEALIAKV
PPPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIRMMATGAQYPVEHIGVF
TPKSKNLESLSAGQVGFIIAGIKELAAAKVGDTVTLVNAPAAEPLPGFKEVKPQVFAGLY
PVEANQYDALRDSLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
MDLITTAPTVVYEVLQRDGTTIMVENPAKMPEPSKIEEVREPIVTVNLYMPQDYVGSVIT
LCTQKRGNQINMQYHGRQVQLTYEIPMGEVVLDFFDRLKSISRGYASMDYEFKEYRASDV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMRELIPRQMYDVAIQATIGSNIIARE
NIKALRKNVLAKCYGGDISRKKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK