Protein Info for H281DRAFT_04229 in Paraburkholderia bryophila 376MFSha3.1

Annotation: glycerol kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR01311: glycerol kinase" amino acids 4 to 495 (492 residues), 748.1 bits, see alignment E=1.7e-229 PF00370: FGGY_N" amino acids 5 to 251 (247 residues), 284.3 bits, see alignment E=8.8e-89 PF02782: FGGY_C" amino acids 261 to 449 (189 residues), 142 bits, see alignment E=2.2e-45

Best Hits

Swiss-Prot: 96% identical to GLPK_PARXL: Glycerol kinase (glpK) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 98% identity to bgf:BC1003_2904)

MetaCyc: 61% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MDL5 at UniProt or InterPro

Protein Sequence (499 amino acids)

>H281DRAFT_04229 glycerol kinase (Paraburkholderia bryophila 376MFSha3.1)
MQDQYILALDQGTTSSRAMLFDRLGNVVSTAQKEFEQIYPRPGWVEHNPQEIWSTQAGVA
AEAVTRAGMNGTSIAAIGITNQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKEQGL
EAKVRAKTGLPIDSYFSATKIRWILDNVEGAREKAKQGRLAFGTVDSWLVWNFTKGGLHV
TDVTNASRTMLFNIHTLKWDDELLDALDIPRSMLPEVRPSSEVYGPTKTTVFASKIPLAG
IAGDQHAALFGQMCTESGMVKNTYGTGCFLVMNTGDKPIESKNNLVTTIAWQIGDQINYA
LEGSIFIGGAVVQWLRDGLGIIKNAAEIETLARGVPHCDGVYLVPAFAGLGAPHWNARAR
GTLFGVTRGTTSAHIARAALDSIAYQSLDVLKAMEADSGIRIGELRVDGGACANNLLMQF
QADILGVDAVRPKVSETTALGAAYLAGLAVGYWKDVNELQSQWKLDRRFTPALEQADVEE
CLDGWKRAIRAAKAWADTP