Protein Info for H281DRAFT_04181 in Paraburkholderia bryophila 376MFSha3.1

Annotation: D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details transmembrane" amino acids 47 to 66 (20 residues), see Phobius details PF02113: Peptidase_S13" amino acids 96 to 527 (432 residues), 289.7 bits, see alignment E=1.9e-90 TIGR00666: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase" amino acids 113 to 510 (398 residues), 209.5 bits, see alignment E=3.5e-66

Best Hits

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 88% identity to bug:BC1001_2916)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>H281DRAFT_04181 D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) (Paraburkholderia bryophila 376MFSha3.1)
MTHAFLSFAARHLHRAALRSRLTSSFIAPLSACCGSAAPRRAASHASAWLLVCAALAGGA
SLPLAAQARVKAAHPSVNVSTVLPQSVMIGLQRAHVPLSSISVVIEKVGDRTPIVALNAG
KPMMPASTMKLVTTYSGLSILGPDYRWRTNAYADGTVDANGVLHGNLYIQGTGDPKLVPE
ELIDLVQKIHKAGIRGIDGALVLDKRYFDPSTRDLPPFDDDASAPYNVGPDPLLYAFKSL
SFTLTPSSDGTVTIDVLPALAQLQIENQMRAVNGPCRGEAASVSPTVTPTPNGTLVASFA
GDYSVRCGPRTVNVAVLDHSAFFAGGFLALWQQTGGTFSGATREGAVPVGAKLVATHQGP
VLSDIVRDINKFSNNTMARNLFLSIGAAEEKGPATPAKSARAIEAFLRRDSIDMEYLTLD
NGSGLSREEHITALSLADLLQRANASPVAQVFVQSLPIAGVDGTMRNRLTNQGAGGNAQI
KTGTLRDVRAIAGYVASADGNSYVVVSFINDPHSEAARAAHDALLEWVYQGPSSGFSKIS
APAAESAGVTPHGKPRKNPRKREAH