Protein Info for H281DRAFT_04171 in Paraburkholderia bryophila 376MFSha3.1

Annotation: membrane fusion protein, multidrug efflux system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF00529: CusB_dom_1" amino acids 35 to 359 (325 residues), 31 bits, see alignment E=4e-11 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 373 (337 residues), 263.3 bits, see alignment E=1.3e-82 PF16576: HlyD_D23" amino acids 62 to 291 (230 residues), 44.8 bits, see alignment E=1.9e-15

Best Hits

Swiss-Prot: 51% identical to ACRA_ECOLI: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli (strain K12)

KEGG orthology group: K03585, membrane fusion protein (inferred from 97% identity to bgf:BC1003_2843)

MetaCyc: 51% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MDG2 at UniProt or InterPro

Protein Sequence (410 amino acids)

>H281DRAFT_04171 membrane fusion protein, multidrug efflux system (Paraburkholderia bryophila 376MFSha3.1)
MRVERVPFRLISAATAAILLAACGPKQSAPPQQTPEVGVVTVQPTTVPVVTELPGRTSAF
LVAQVRARVDGIVLRREFTEGGQVKAGQRLYKIDPAPYIATLNNAKATLAKAQANLASTT
AQASRFKVLVAANAVSKQDYDNAVAAQGQAAADVAAGKAAVETAQINLGYTDVTSPVTGQ
IGVSQVTPGAYVQASAATLLATVQQLDPVYVDLTQSSLEGLKLRRQMQEGRLKTNGPDAA
KVSLILEDGRTYSEKGKLQFTDVTVDQGTGSVTVRAIFQNKDKVLLPGMFVRAKIEEGVN
ENALVVPQVGITHDQKGQPTALVVGEDNKVALRQLVTSGTYGSNWVVESGLKPGDRVIVQ
GTDKAKPGMQVKTVAAQLPATPASGAAAQGAPAASGSAQTAQPASAASGA