Protein Info for H281DRAFT_04147 in Paraburkholderia bryophila 376MFSha3.1

Annotation: L-iditol 2-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08240: ADH_N
  • amino acids 37 to 154 (118 residues), 98.5 bits, E=2e-32
• PF00107: ADH_zinc_N
  • amino acids 197 to 325 (129 residues), 81.7 bits, E=4.7e-27

Best Hits

Swiss-Prot: 50% identical to ELTD_MYCS2: Erythritol/L-threitol dehydrogenase (eltD) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: K00008, L-iditol 2-dehydrogenase [EC: 1.1.1.14] (inferred from 96% identity to bpy:Bphyt_3233)

MetaCyc: 50% identical to erythritol/L-threitol dehydrogenase (Mycolicibacterium smegmatis)
1.1.1.M29 [EC: 1.1.1.M29]; 1.1.1.M28 [EC: 1.1.1.M29, 1.1.1.M28]; D-xylulose reductase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9]; Ribitol 2-dehydrogenase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9, 1.1.1.56]; L-arabinitol 4-dehydrogenase. [EC: 1.1.1.M29, 1.1.1.M28, 1.1.1.9, 1.1.1.56, 1.1.1.12]

Predicted SEED Role

"Xylitol dehydrogenase (EC 1.1.1.9)" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 1.1.1.9)

MetaCyc Pathways

• D-sorbitol degradation I (3/3 steps found)
• xylitol degradation I (2/2 steps found)
• L-arabinose degradation II (2/3 steps found)
• ribitol degradation I (1/2 steps found)
• erythritol degradation II (1/3 steps found)
• L-threitol degradation (1/4 steps found)
• superpathway of pentose and pentitol degradation (21/42 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.14

Use Curated BLAST to search for 1.1.1.12 or 1.1.1.14 or 1.1.1.56 or 1.1.1.9 or 1.1.1.M28 or 1.1.1.M29

Protein Sequence (365 amino acids)

>H281DRAFT_04147 L-iditol 2-dehydrogenase (Paraburkholderia bryophila 376MFSha3.1)
MTNQSTSSDQQNMTAIVCHAPKDYRVEQVAKPRAGAHELVIRIAACGICASDCKCHSGAK
MFWGGPSPWVKAPVIPGHEFFGFVQELGDGAAEHFGVKVGDRVIAEQIVPCAKCRYCKSG
QYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISLEDAAIIEPLACAIHTVN
RGELQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLVEERLALAREYGADVTINPKT
DDALAIIHSLTDGYGCDVYIETTGAPIGVNQGMDLIRKLGRFVEFSVFGADTTLDWSVIG
DRKELDVRGAHLGPYCYPIAIDLLARGLVTSKGIVTHGFSLEEWDEAIKIANSLDSIKVL
MKPRA