Protein Info for H281DRAFT_04125 in Paraburkholderia bryophila 376MFSha3.1

Annotation: Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 91% identity to bug:BC1001_2860)

Predicted SEED Role

"O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)" in subsystem Menaquinone and Phylloquinone Biosynthesis (EC 6.2.1.26)

MetaCyc Pathways

superpathway of chorismate metabolism (43/59 steps found)
2-carboxy-1,4-naphthoquinol biosynthesis (1/7 steps found)
superpathway of menaquinol-8 biosynthesis I (3/10 steps found)
superpathway of demethylmenaquinol-8 biosynthesis I (2/9 steps found)
superpathway of menaquinol-10 biosynthesis (2/10 steps found)
superpathway of menaquinol-11 biosynthesis (2/10 steps found)
superpathway of menaquinol-12 biosynthesis (2/10 steps found)
superpathway of menaquinol-13 biosynthesis (2/10 steps found)
superpathway of menaquinol-6 biosynthesis (2/10 steps found)
superpathway of menaquinol-7 biosynthesis (2/10 steps found)
superpathway of menaquinol-9 biosynthesis (2/10 steps found)
superpathway of demethylmenaquinol-6 biosynthesis I (1/9 steps found)
superpathway of demethylmenaquinol-9 biosynthesis (1/9 steps found)
superpathway of phylloquinol biosynthesis (2/15 steps found)

KEGG Metabolic Maps

Ubiquinone and menaquinone biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>H281DRAFT_04125 Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II (Paraburkholderia bryophila 376MFSha3.1)
LSTASPLRSRIDVPALLAALPARISDIPALAAGRDPQHVALIEDGRRLTNAQLVQAVEAT
AALLRDWGVRGGDRVMIVAENSIAQIVLLFATARLDAWALVSNARLSASELDAIRAHAQP
RVTAYVVESSADAGQHAQRHGASAAPDVAPGIGAWSFTVDTFAQAEPVEAANDRQCAALI
YTTGTTGAPKGVMLSHRNLLFIAAVSSRLRQVRPDDVVYAVLPISHVYGFASVCLGSLYA
GATLRLAPRFAPGAVRRALADERVSIFQGVPAMHAKLIEHLQTHGHAWHAPHLRFIYSGG
SPLDAALKAHVESVYGLPLHNGYGMTESSPTVSQTMLDAPRRDCSVGEVIPGVEVRFVGL
DGADVAVGETGELWVRGPNVMLGYYRNPQQTRAAVTQDGWLKTGDLARQDADGALHIVGR
SKDLIIRSGFNVYPAEVEHVLNAHPQVVQSAVIGRPVEGNEEVIAFVELAANASVTPAEL
TAWCVERLAPYKRPAEVKVLAALPAASTGKILKHRLREFL