Protein Info for H281DRAFT_04090 in Paraburkholderia bryophila 376MFSha3.1

Annotation: high-affinity nickel-transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 37 to 55 (19 residues), see Phobius details amino acids 78 to 103 (26 residues), see Phobius details amino acids 116 to 140 (25 residues), see Phobius details amino acids 190 to 214 (25 residues), see Phobius details amino acids 221 to 242 (22 residues), see Phobius details amino acids 263 to 288 (26 residues), see Phobius details amino acids 310 to 332 (23 residues), see Phobius details PF03824: NicO" amino acids 40 to 330 (291 residues), 323.9 bits, see alignment E=4.6e-101 TIGR00802: transition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family" amino acids 43 to 325 (283 residues), 391.9 bits, see alignment E=8.7e-122

Best Hits

Swiss-Prot: 57% identical to HOXN_CUPNH: High-affinity nickel transport protein (hoxN) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K07241, high-affinity nickel-transport protein (inferred from 91% identity to bug:BC1001_2825)

Predicted SEED Role

"HoxN/HupN/NixA family nickel/cobalt transporter" in subsystem Transport of Nickel and Cobalt

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A2Z5MFJ0 at UniProt or InterPro

Protein Sequence (342 amino acids)

>H281DRAFT_04090 high-affinity nickel-transport protein (Paraburkholderia bryophila 376MFSha3.1)
MTPTASLRPRLLMLYTVLIAANLGAWAWALIAFRHYPLLLGTALLAYGFGLRHAVDADHI
AAIDAVTRKLMQTGKRPLGVGLSFSLGHSSIVILATLGIAWTAHSLHGRFETFKAVGGTI
GTLVSATFLLTLAAVNLVILRDVWRRYRHVQRDGELAAAALDTDHAAGPGGLLSRALRPL
FRLVTKSWHMYPVGVLFGLGFDTATEIGLLAIAASEAGKGLPLYSILVFPALFTAGMTLV
DSTDNVLMVHAYGWAMDDPKRKLYYNASITFVSAVVAIVIGGVEALGLLSDKLGLNGGIW
DAVTNANEQFGVLGYGIVAAFMACWIGSVLFHRWKRPAIAAR